Jak (Janus Kinase) and Stat (signal transducer and activator of transcription) proteins are utilized by receptors for a wide varity of ligands including cytokines, hormones, growth factors, and neurotransmitters. Jaks and Stats play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses, and stem cell differentiation. Therefore, regulation of Jak/Stat signaling is crucial to prevent aberrant signaling which can lead to disease progression. Two methods for regulating Jak/Stat signaling involve SOCS and PIAS proteins.The SOCS (suppressor or cytokine signaling) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway and consist of 8 known members, including the originally identified protein CIS1 (cytokine-inducible SH2-containing protein) and SOCS1-SOCS7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. SOCS proteins are involved in regulating over 30 cytokines, including interleukins, growth hormone (GH), interferons, leptin, and leukemia inhibitory factor. SOCS1, also known as JAB (Janus Kinase binding protein) and SSI-1 (Stat-induced Stat inhibitor-1), shares the most homology with SOCS3 and both are highly induced by cytokines. Both SOCS1 and SOCS3 directly inhibit Jak activity; SOCS1 inhibits Jak through an interaction involving a phospohotyrosine located in the kinase activation loop; SOCS3 inhibits Jak via its SH2 domain. In addition to inhibiting Jak/Stat signaling, the SOCS box of SOCS1 and SOCS3 can trigger ubiquitin-mediated degradation of proteins within and outside the Jak/Stat pathway. SOCS2 is also incduced upon cytokine stimulation and the activity of SOCS2 has been predominately linked to GH and insulin-like growth factors.
Product List
| Target | Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|---|
| SOCS1 | APRab03838 | SOCS1 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC-F,IHC-P,ICC/IF,ELISA |
| SOCS-1 | APRab18089 | SOCS-1 Rabbit Polyclonal Antibody | Human,Mouse,Rat | IF-P,IF-F,ICC/IF,WB,IHC-P,ELISA |
| SOCS-2 | APRab18091 | SOCS-2 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC-P |
| SOCS-3 | APRab18092 | SOCS-3 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC-P,IF-P,IF-F,ICC/IF |
| SOCS3 | AMM82829 | SOCS3 Mouse Monoclonal Antibody | Human | WB,IHC,ICC,FC,ELISA |
| SOCS2 | AMRe84294 | SOCS2 Rabbit Monoclonal Antibody | Human | WB,IP,ICC |
| SOCS7 | AMRe84701 | SOCS7 Rabbit Monoclonal Antibody | Human | WB,IHC |
| PIAS1/2 | AMRe03265 | PIAS1/2 Rabbit Monoclonal Antibody | Human | WB,IHC-P,IP |
| PIAS 3 | APRab16120 | PIAS 3 Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| PIAS 1 | APRab16119 | PIAS 1 Rabbit Polyclonal Antibody | Human,Mouse | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| PIASx | APRab16121 | PIASx Rabbit Polyclonal Antibody | Human,Mouse,Rat,Monkey | WB,ELISA,IHC-P |
| PIASy | APRab16122 | PIASy Rabbit Polyclonal Antibody | Human,Mouse | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| PIAS1+PIAS2 | AMRe83949 | PIAS1+PIAS2 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IP,IHC,ICC,FC,IF |
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References
- Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Darnell JE Jr, et al. Science. 1994. [PMID: 8197455]
- Human IL-3 stimulates endothelial cell motility and promotes in vivo new vessel formation. Dentelli P, et al. J Immunol. 1999. [PMID: 10438956]
- Signalling through the JAK-STAT pathway in the developing brain. Cattaneo E, et al. Trends Neurosci. 1999. [PMID: 10407422]
- Rapid STAT phosphorylation via the B cell receptor. Modulatory role of CD19. Su L, et al. J Biol Chem. 1999. [PMID: 10542198]
- Regulation of cytokine signaling pathways by PIAS proteins. Shuai K. Cell Res. 2006. [PMID: 16474434]
- SOCS regulation of the JAK/STAT signalling pathway. Croker BA, et al. Semin Cell Dev Biol. 2008. [PMID: 18708154]
- Suppressors of cytokine signaling (SOCS): negative regulators of signal transduction. Alexander WS, et al. J Leukoc Biol. 1999. [PMID: 10534114]
- SOCS proteins, regulators of intracellular signaling. Chen XP, et al. Immunity. 2000. [PMID: 11021526]
- Twenty proteins containing a C-terminal SOCS box form five structural classes. Hilton DJ, et al. Proc Natl Acad Sci U S A. 1998. [PMID: 9419338]
- A family of cytokine-inducible inhibitors of signalling. Starr R, et al. Nature. 1997. [PMID: 9202125]
- The JAK-binding protein JAB inhibits Janus tyrosine kinase activity through binding in the activation loop. Yasukawa H, et al. EMBO J. 1999. [PMID: 10064597]
- Cytokine-inducible SH2 protein-3 (CIS3/SOCS3) inhibits Janus tyrosine kinase by binding through the N-terminal kinase inhibitory region as well as SH2 domain. Sasaki A, et al. Genes Cells. 1999. [PMID: 10421843]
- The SOCS box of SOCS-1 accelerates ubiquitin-dependent proteolysis of TEL-JAK2. Kamizono S, et al. J Biol Chem. 2001. [PMID: 11278610]
- SOCS-1 and SOCS-3 block insulin signaling by ubiquitin-mediated degradation of IRS1 and IRS2. Rui L, et al. J Biol Chem. 2002. [PMID: 12228220]
- Interaction of human suppressor of cytokine signaling (SOCS)-2 with the insulin-like growth factor-I receptor. Dey BR, et al. J Biol Chem. 1998. [PMID: 9727029]
- Inhibition of Stat1-mediated gene activation by PIAS1. Liu B, et al. Proc Natl Acad Sci U S A. 1998. [PMID: 9724754]
- Specific inhibition of Stat3 signal transduction by PIAS3. Chung CD, et al. Science. 1997. [PMID: 9388184]
- PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4. Arora T, et al. J Biol Chem. 2003. [PMID: 12716907]
- PIASy controls ubiquitination-dependent proteasomal degradation of Ets-1. Nishida T, et al. Biochem J. 2007. [PMID: 17456046]
- PIASy represses CCAAT/enhancer-binding protein delta (C/EBPdelta) transcriptional activity by sequestering C/EBPdelta to the nuclear periphery. Zhou S, et al. J Biol Chem. 2008. [PMID: 18477566]
- The role of JAK/STAT signaling pathway and its inhibitors in diseases. Xin P, et al. Int Immunopharmacol. 2020. [PMID: 31972425]
- Molecular pathways: Jak/STAT pathway: mutations, inhibitors, and resistance. Quintás-Cardama A, et al. Clin Cancer Res. 2013. [PMID: 23406773]
- JAK/STAT inhibitors mitigate sepsis-associated cerebral and cognitive injury. Bhadauriya MS, et al. Fundam Clin Pharmacol. 2025. [PMID: 40192210]
