EnkiLife | BacuVance™ Insect Protein Expression Service

Insect Protein Expression Service

High-yield baculovirus expression in Sf9, Hi5, and Sf21 cells for complex eukaryotic proteins.

Specialized in transmembrane proteins, kinases, high-MW proteins, and multi-subunit complexes. Full milestone workflow from gene synthesis to purified protein.

View Workflow Key Features

Baculovirus expression system Advantages

High success rate for transmembrane proteins (1-7 TMDs)
Optimized for kinases, high-MW, and multi-subunit complexes
Free codon optimization for Sf9, Sf21, Hi-5cells

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>85% Success Rate

For >200 membrane proteins since 2020

Service Details & Milestone Workflow

Milestone	Activities & Specifications	Deliverables	Timeline
1. Gene Synthesis & Subcloning	Codon optimization for insect cells Subcloning into expression vectors (e.g., pFastBacI)	Sequence-verified construct(s) report	2 weeks
2. Virus Generation	Recombinant Bacmid DNA generation Transfection, P1 and P2 virus stock Titer determination	MOI evaluation report Recommended optimal condition, or >10⁷ pfu/mL virus stock	2-3 weeks
3. Pilot Expression Evaluation	Infection with P2 stock One-step affinity purification SDS-PAGE and Western blot	Expression data & optimization report	2-3 weeks
4. 1 L Expression & Purification	Scale-up to 1 L culture One-step or two-step purification QC: SDS-PAGE, WB, LC-MS/MS	Purified protein, QC data, CoA	2-3 weeks