Product Information

The extraction of protein from cells or tissues is one of the key factors that affect the results of Western blotting analysis (WB).In practice,the extraction of protein

 from cell membranes, cytoplasm, organelles, and nuclei usually uses detergent-based buffers such as radioimmunoprecipitation assay (RIPA) buffer, physical 

disruption such as sonication, or a combination of both, in particular, RIPA buffer containing 0.1% SDS or its substitutes (such as NP-40 buffers without SDS) have 

been widely used as a standard method for the lysis of mammalian cells and tissues.In fact, RIPA can effectively dissolve and extract most of the proteins of 

medium and small molecules with a molecular weight of less than 90 kDa, but it is not effective in extracting large proteins with a molecular weight of greater 

than 90 kDa. In order to extract large proteins more effectively, many laboratories use a combination of RIPA buffer and sonication to physically break down DNA 

to reduce the viscosity of the lysates. However, sonication has the potential to break down large proteins. In addition, in order to  inhibit endogenous enzyme 

activities, inhibitors need to be added to the RIPA buffer.For example, to reduce protein degradation, protease inhibitors such as aprotinin, leupeptin, pepstatin, 

and PMSF need to be added to RIPA buffer immediately before use. Similarly, sodium fluoride and sodium orthovanadate must be added to inhibit phosphatase 

activities. 

Our Cell/Tissue Lysis buffer solve these issues. It can quickly and completely extract proteins from cells and tissues,avoiding adding protease, phosphatase, and 

other enzyme inhibitors; it can also preserve the post-translational modifications (PTMs) of the cellular proteins. Overall, this product is suitable for extracting 

proteins of all sizes from mammalian cells and tissues.

Key Features

1.No proteases/other enzyme inhibitors or sonication required. 

2.Simply mix A and B;Extraction takes only 15 min

3.Extract large proteins nearly completely; no sonication to avoid protein fragmentation.

4.No loss of protein PTMs such as phosphorylation, glycosylation, ubiquitination, methylation, and acetylation

5.Suitable for mammalian cells and tissues.

Storage

Store Reagent A at -20°C. Store Reagent B at room temperature or 4°C

Note:Precipitation may occur when Reagent B is stored at 4 °C over a long time, but it does not affect product quality. The precipitation will redissolve at room 

temperature.

Application

Denaturing protein extraction; Western blotting