MRN Complex
The Mre11-Rad50-Nbs1 (MRN) complex is a key mediator of genome maintenance, playing important roles in meiosis, telomere stability at the ends of chromosomes, and the cellular responses to DNA damage. Homodimers of the Mre11 and Rad50 subunits form a tetramer core that binds directly to DNA and associates with the Nbs1 subunit.
The complex functions as a sensor of DNA damage and localizes to DNA double-strand breaks. At these DNA lesions, the MRN complex tethers DNA ends and processes free strands via the endonuclease and exonuclease activities of Mre11. In addition to stimulating both homologous recombination and nonhomologous end joining repair DNA pathways, MRN activates DNA damage checkpoint signaling cascades regulating cell cycle progression.
In some contexts, MRN is required for ATM activation and downstream phosphorylation of p53, BRCA1, and Chk2. ATM also phosphorylates Mre11, Rad50, and Nbs1 (also known as p95 and Nibrin). Notably, Nbs1 Ser343 and Mre11 Ser676 are phosphorylated by ATM. Phosphorylation modulates function and association with many mediators, some of which include 53BP1, RPA, hSSB1, TRF2, BRCA1, FANCD2, CtP1, Histone H2AX, MDC1, and WRN helicase.
Each subunit is essential for mammalian embryonic development, as mice with homozygous-null mutations in Mre11, Nbs1, or Rad50 are lethal. Furthermore, MRN complex function is required in developing lymphocytes for antigen receptor gene recombination initiated by the Rag-1 and Rag-2 recombinases. In humans, Mre11 and Nbs1 mutations cause chromosomal instability and radiosensitivity and are associated with ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS), respectively. Genomic instability and cancer have been shown to develop in cells with genetic mutations within MRN complex genes.
The multiple roles of the MRN complex in cell homeostasis.
Relevant antibodies
| Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|
| AMRe14083 | Mre11 (9C7) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC-P,FC,IF-P |
| AMRe03176 | Rad50 Rabbit Monoclonal Antibody | Human | WB,IHC-P |
| AMRe05962 | Phospho-p95/NBS1 (S343) (8Q6) Rabbit Monoclonal Antibody | Human | WB,ICC/IF |
| APRab14692 | Nibrin Rabbit Polyclonal Antibody | Human,Rat,Mouse | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | — | — |
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody | — | — |
Related Products
References
- D'Amours D, Jackson SP. The Mre11 complex: at the crossroads of dna repair and checkpoint signalling. Nat Rev Mol Cell Biol. 2002 May;3(5):317-27.[PMID: 11988766].
- Ajimura M, Leem SH, Ogawa H. Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae. Genetics. 1993 Jan;133(1):51-66.[PMID: 8417989].
- Petroni M, La Monica V, Fabretti F, Augusto M, Battaglini D, Polonara F, Di Giulio S, Giannini G. The Multiple Faces of the MRN Complex: Roles in Medulloblastoma and Beyond. Cancers (Basel). 2023 Jul 13;15(14):3599.[PMID: 37509263].
 | Flora Flora is a technical support expert at EnkiLife, familiar with immunology and neuroscience, dedicated to providing customers with high-quality product combinations and technical support to help achieve research in neurodegenerative diseases and other neuroscience areas. |