Cleaved Caspase
Caspase Family: Caspases are a class of cysteine proteases and key executors of apoptosis (programmed cell death). Under normal conditions, they exist in an inactive zymogen form. When cells receive apoptotic signals, these zymogens are activated into active enzymes.
Formation of Cleaved Caspase: During apoptosis, caspase zymogens are cleaved to form active enzymes. This cleavage is irreversible and marks the irreversible stage of apoptosis.In many diseases, abnormal regulation of apoptosis is closely related to the occurrence and development of the disease. For example, in cancer, cancer cells may escape apoptosis by inhibiting the activation of Caspase; in neurodegenerative diseases, excessive apoptosis may lead to the death of nerve cells.
Types of Caspases
• Caspase-3: It is the most classic apoptotic executioner enzyme. Its cleavage fragments are commonly used as markers of cell apoptosis.
• Caspase-8: It mainly participates in the extrinsic apoptotic pathway, which is activated through death receptors (such as Fas).
• Caspase-9: It mainly participates in the intrinsic apoptotic pathway, which is activated by cytochrome C released from mitochondria.
Relevant Antibodies
| Catalog# | Product Name | Application | Reactivity |
|---|---|---|---|
| APRab08957 | Cleaved-Caspase-3 p12 (D175) Rabbit Polyclonal Antibody | WB,IF-P,IF-F,ICC/IF,IHC-P,ELISA | Human,Rat,Mouse |
| APRab00828 | Cleaved-Caspase 6 p18 Rabbit Polyclonal Antibody | WB,IHC-P,ELISA | Human,Rat |
| APRab08967 | Cleaved-Caspase-7 p20 (D198) Rabbit Polyclonal Antibody | WB,ELISA | Human,Mouse |
| APRab08970 | Cleaved-Caspase-9 (D330) Rabbit Polyclonal Antibody | IF-P,IF-F,ICC/IF,WB,IHC-P,ELISA | Human,Rat,Mouse |
| APRab08971 | Cleaved-Caspase-9 p35 (D315) Rabbit Polyclonal Antibody | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA | Human,Rat,Mouse |
| APRab09025 | Cleaved-PARP-1 (D214) Rabbit Polyclonal Antibody | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA | Human,Mouse |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | ELISA, WB, Dot blot | Rabbit |
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody | WB,ELISA | Human,Mouse,Rat,Rabbit,Dog,Monkey |
Related Products
Super-sensitive ECL chemiluminescent reagent
References
Fernandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J Biol Chem. 1994 Dec 9;269(49):30761-4. [PMID: 7983002].
Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant M, Gareau Y, Griffin PR, Labelle M, Lazebnik YA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 1995 Jul 6;376(6535):37-43. [PMID: 7596430].
 | Flora Flora is a technical support expert at EnkiLife, familiar with immunology and neuroscience, dedicated to providing customers with high-quality product combinations and technical support to help achieve research in neurodegenerative diseases and other neuroscience areas. |