EnkiLife | Cell-Free Protein Expression Service (E. coli system)

Cell-Free Protein Expression Service

Rapid, open-system expression for challenging proteins: toxic, multi-transmembrane, or unstable targets.

Bypass cell culture and transfection. Produce proteins in days, not weeks, with optimized E. coli extracts and reaction buffers. Ideal for high-throughput screening and proteins that are difficult to express in vivo.

View Workflow Platform Features

Platform Features & Advantages

⚡ Efficient

Optimized E. coli extract with simplified operation. No cloning or transformation delays – add template directly to reaction.

📅 Short Cycle

Expression completed within one week. From gene synthesis to purified protein in as little as 10-12 days total.

🛡️ Expression Compatibility

Capable of expressing proteins toxic to host cells, including multiple transmembrane proteins (e.g., 7-TMD targets).

Service Details & Milestone Workflow

Step	Activity	Deliverables	Timeline
1. Molecular Design	Gene optimization & synthesis for cell-free system Subcloning into vector with T7 promoter (or linear template) Optional: fusion tags	Optimized gene construct	5-10 days
2. Expression Testing	Preparation of 1 mL system Optimization of reaction conditions SDS-PAGE detection	Expression test report	2-3 days
3. Scale-Up	Scale-up to 1–10 mL reactions Purification via affinity chromatography	Purified protein	1-2 days
4. Sample Testing & QC	Purity detection (SDS-PAGE, SEC-HPLC) Concentration measurement Optional: Western blot, activity assay	QC data package	1-2 days