Antibody Sequencing Services
From hybridoma to sequence in 1 week.
Accurate determination of antibody variable and full-length sequences from hybridoma cells, B cells, or single B cells. Choose Sanger sequencing or mass spectrometry for protein-level de novo sequencing and PTM analysis. Species: human, mouse, rat, rabbit, and more.
Why Sequence from Hybridoma Cells?
🔍 Preserved Diversity
Captures natural antibody diversity from original B cell clone.
✅ Quality Control
Ensures sequenced antibody matches functional hybridoma clone.
🚀 Path to Recombinant Ab
Enables recombinant expression and antibody engineering.
🔒 IP Protection
Strict confidentiality for all sequence data.
Antibody Sequencing Workflow
Two workflows tailored to your sample type and research goals.
Sanger Sequencing
Sample: Hybridoma cells, B cells, or single B cells (>1×10⁶ cells)
RNA Extraction
Isolate total RNA
RT-PCR
Amplify variable & full-length regions
Sanger Sequencing
Capillary electrophoresis
Bioinformatics
CDR/FR annotation, V(D)J assignment
Delivery
Sequences + analysis report
Mass Spectrometry Sequencing
Sample: Purified antibody protein (≥50 µg)
Protein Purification
Purify from supernatant
Proteolytic Digestion
Generate peptide fragments
LC-MS/MS
Peptide separation & fragmentation
De Novo & PTM
Sequence assembly, PTM identification
Delivery
Full sequence + PTM report
Service Packages
| Service Package | Deliverables | Optional Add-ons |
|---|---|---|
| Sanger Sequencing Variable Domain / Full-Length Antibody |
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| Mass Spectrometry Sequencing De Novo Protein Sequencing |
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Sanger vs Mass Spectrometry
Sanger Sequencing
Best for: Individual hybridoma clones, nucleotide-level information.
- High accuracy for single sequences
- Provides nucleotide sequence (enables recombinant expression)
- Full-length coding sequence available
- Requires RNA/cell sample
Mass Spectrometry
Best for: Protein-level confirmation, PTM analysis, when RNA is unavailable.
- Direct analysis of antibody protein
- Identifies post-translational modifications
- De novo sequencing without prior information
- Works with purified antibody samples
Selection Criteria
| Factor | Sanger Sequencing | Mass Spectrometry |
|---|---|---|
| Sample Type | Cells (hybridoma, B cells) | Purified antibody protein |
| Output | Nucleotide + deduced amino acid | Amino acid sequence (protein-level) |
| PTM Detection | Cannot detect | Comprehensive PTM identification |
| Recombinant Expression | Directly enables | Requires reverse translation |
| Best When | Need sequence for cloning; have hybridoma cells | Protein sample only; need PTM confirmation |
Simple Quotation & Ordering
Consultation
TAM contacts you within 24h for strategy and quote.
Approval
Review and approve final order details and price.
Project Start
Project initiated; PM provides updates until delivery.
Start Your Monoclonal Project
To discuss your target and receive a customized proposal, please email order@enkilife.com. Our team is ready to assist you right away.