Mammalian Protein Expression Service

High-yield recombinant protein production in HEK293 and CHO systems.

From μg to g scale, with native post-translational modifications. Ideal for therapeutic candidates, target screening, and complex eukaryotic proteins. Fast turnaround time from just two weeks.

View Workflow Key Features

Mammalian system Advantages

high-throughput and large-scale services.
High yield, from μg to g.
Full PTMs: glycosylation, phosphorylation, complex folding
Multiple isotypes available (IgG, IgM, etc.)for recombinant antibody express

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Fast Track Available

Express optimization in weeks, not months.

Service Details & Milestone Workflow

Our mammalian expression service follows a streamlined, milestone-driven process.

Milestone	Activities & Specifications	Deliverables	Timeline
1. Gene Design & Vector Construction	Codon optimization for CHO/HEK293 Subcloning into mammalian vector (e.g., pcDNA3.4) Optional: signal peptide, tags	Optimized gene sequence report Sequence-verified plasmid Maxiprep DNA	1-2 weeks
2. Transfection & Small-Scale Expression	Transient transfection in suspension cells Small-scale culture (30-100 mL) Initial titer estimation	Expression evaluation report Titer data	1-2 weeks
3. Scale-Up Production	Scale-up to desired volume (1 L – 1000 L) Fed-batch or perfusion optimization Harvest by centrifugation/filtration	Conditioned medium or cell pellet Production batch record	2-4 weeks
4. Purification & Polishing	Affinity chromatography (Protein A or tag-specific) Optional: IEX, SEC, or HIC polishing Optional: viral inactivation, endotoxin removal	Purified protein in formulation buffer Yield and concentration	1-2 weeks
5. Quality Control & Delivery	SDS-PAGE, SEC-HPLC (purity) Optional: LC-MS (intact mass, glycosylation) Optional: Endotoxin, bioburden testing	Certificate of Analysis QC data package Aliquoted protein	1 week