Yeast Protein Expression Service

EnkiLife | Yeast Protein Expression Service (Pichia/S. cerevisiae)

Yeast Protein Expression Service

Cost-effective, scalable, and high-yield recombinant protein production in yeast.

Flexible host options (Pichia pastoris and Saccharomyces cerevisiae), integrated gene-to-protein solutions, suitable for industrial-scale fermentation.

View Workflow                Why Yeast?
Yeast Expression

Why Choose Yeast Expression?

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Eukaryotic protein folding and processing

Produces glycosylation structures similar to higher eukaryotes (8-14 mannose residues per branch),high protein solubility

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Mature system

Clearly designed genetic background, easy to operate.

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Cost-Effective & Scalable

Fast growth with high yields, enabling efficient pilot studies and cost-effective scale-up to grams or more.

Service Details & Milestone Workflow

StepActivities & SpecificationsDeliverablesTimeline
1. Expression Vector Construction
  • Codon optimization for yeast
  • Gene synthesis
  • Subcloning into yeast vector (pPICZ, pGAPZ, pYES)
Sequence-verified construct1-2 weeks
2. Small-Scale Expression & Clone Screening
  • Transformation and selection
  • Identification of top-expressing clones
  • Expression optimization
Top clone, expression data2-3 weeks
3. Scale-Up & Purification
  • Fermentation (shake flask or bioreactor)
  • Chromatography purification
  • Comprehensive QC
Purified protein, QC data2-4 weeks

Service Portfolio & Timeline

  • Full Service 5-7 weeks
  • Protein Pilot 4-5 weeks
  • Scale-Up ~2 weeks after pilot

*Additional QC and custom requirements may extend timelines.

Related Expression Systems

Beyond yeast, we offer a full portfolio of expression hosts to match your protein's unique needs.

Mammalian

Mammalian System

Full PTMs, ideal for therapeutic candidates and complex eukaryotic proteins.

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Insect

Insect (Baculovirus)

Glycosylated proteins, secreted forms, complex eukaryotic proteins.

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E. coli

E. coli System

Fast, high-yield for antigens, enzymes, and proteins without PTM requirements.

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Simple Quotation & Ordering

1

Submit Request

Email order@enkilife.com with protein details.

2

Consultation

TAM contacts you within 24h for strategy and quote.

3

Approval

Review and approve final order details and price.

4

Project Start

Project initiated; PM provides updates until delivery.

Contact Us

To discuss your custom protein needs, please email order@enkilife.com. Our team is ready to assist you right away.

⬇ Download Custom Protein Request Form
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