EnkiLife | Bacterial Protein Customized Service

Bacterial Protein Customized Service

Optimized expression in E. coli for rapid, high-yield, and cost-effective protein production.

From gene synthesis to purified protein, we offer tailored solutions for antigens, enzymes, cytokines, and more. Specialized strategies for soluble expression and inclusion body refolding.

View Workflow Key Features

Key Advantages of E.coli system

💰 Cost-effective

Rapid expression with short cultivation time and easy operation.

📈 Easy to scale up production

Fermentation for large-scale production with minimal batch variations.

🧬 Mature system

Clearly designed genetic background, streamlined operational procedures.

🔬 Testing conditions

Cultivation temperature, induction conditions; host selection, and soluble protein strategies.

Service Details & Milestone Workflow

Milestone	Activities & Specifications	Deliverables	Timeline
1. Gene Synthesis, Cloning & Plasmid Prep	Gene synthesis & codon optimization Subcloning into expression vector Plasmid prep & DNA QC	Optimized gene sequence report Sequence-verified clone QC data	1-2 weeks
2. Protein Expression Evaluation	Transformation into multiple strains Small-scale expression testing Optimization of induction conditions	Expression evaluation report Recommended optimal condition	1-2 weeks
3. Protein Purification & Processing	Scale-up expression Purification (affinity, IEX, SEC) Optional: Tag removal, refolding	Purified protein in specified buffer Yield and concentration data	2-4 weeks
4. QC & Delivery	Purity analysis (SDS-PAGE, SEC-HPLC) Optional: Identity confirmation (WB, MS) Quantitation (Bradford, A280)	Certificate of Analysis QC data package Protein in aliquots	1 week