Custom Monoclonal Antibody Development
High-specificity monoclonal antibodies.
End-to-end mAb development: from antigen design and immunization to hybridoma development, subcloning, and application-specific screening. Ideal for WB, IHC, IP, FC, and ELISA. Guaranteed positive clones and fast turnaround.

Why Choose Monoclonal Antibodies?
- Unparalleled Specificity – Recognizes a single epitope, eliminating cross-reactivity and ensuring precise targeting in complex assays like IHC, FC, and ELISA.
- Consistent & Reproducible – Infinite supply of identical antibodies provides batch-to-batch uniformity, critical for long-term studies and diagnostic applications.
- High Sensitivity with Low Background – Clonal selection yields high-affinity binders with minimal non-specific binding, ideal for detecting low-abundance targets.
- Custom Design & Screening – Development includes functional screening (e.g., neutralizing, phosphorylation-specific) to match exact application needs.
- Scalable Production – Hybridoma or recombinant platforms enable stable, large-scale manufacturing with defined genetic sequences.

Monoclonal Antibody Development Packages
Flexible packages for mouse, rabbit, and rat hosts. All include hybridoma development, subcloning, and QC.

| Milestone | Activities & Specifications | Deliverables | Timeline |
|---|---|---|---|
| Antigen Preparation (Optional) | Protein: customer provided or EnkiLife expressed Peptide: customer provides or EnkiLife synthesized | Remaining unused antigen delivered at project completion | 1-2 weeks (Protein expression: project dependent) |
| Immunization, Fusion & Screening | Immunization (4-6 weeks), fusion, HAT selection, ELISA screening | 20+ ELISA-positive supernatants | 6-10 weeks |
| Subcloning & Cryopreservation | 2 rounds of limited dilution subcloning, expansion, freezing | 3 monoclonal hybridoma lines per target | 5-8 weeks |
| Purification & QC | Small-scale production, protein A/G purification, QC testing | Purified antibody (0.1-1 mg), CoA | 2-3 weeks |
Immunogen Requirements (customer-provided protein)
- Purity ≥ 75%
- Quantity ≥ 1.5 mg
- Concentration ≥ 0.4 mg/ml
- Homology ≤ 90%
- MW >10 KDa
- Tags His/Flag accepted
⚬ For additional customization (e.g., larger scale, sequencing) please inquire.
Additional & Related Services
Explore our complete antibody platform and technical guides.

Small Molecule & PTM Antibody Development
Targeted small molecule/phosphorylation-state screening,PTM antibody counter-screening.
Learn More
Anti-idiotype Antibody Services
For PK/PD and immunogenicity testing. Developed against your therapeutic antibody.
Learn More
Rabbit Single B Cell Platform
Directly clone rabbit IgG sequences from single B cells,expression in CHO/HEK293 systems, bypassing hybridoma fusion.
Learn More
Sequencing & Engineering Antibody
Sequencing, cloning, and expression in CHO/HEK293. Humanization, affinity maturation, bispecific design.
Learn MoreSimple Quotation & Ordering
Consultation
TAM contacts you within 24h for strategy and quote.
Approval
Review and approve final order details and price.
Project Start
Project initiated; PM provides updates until delivery.
Start Your Monoclonal Project
To discuss your target and receive a customized proposal, please email order@enkilife.com. Our team is ready to assist you right away.
⬇ Download Antibody Request Form