ECM Profiling
The extracellular matrix (ECM) is a cell surface associated three-dimensional macromolecular network composed of collagens, proteoglycans/glycosaminoglycans, elastin, fibronectin, laminins, and several other glycoproteins. The network provides a dynamic microenvironment surrounding the cell enabling it to carry on its function.
Among the ECM proteins, fibronectin functions as a mediator to bridge distinct ECM components such as collagens, growth factors, as well as cell surface integrins to regulate ECM structural change and initiate signaling pathways. During normal development and pathological conditions, the ECM network is highly dynamic and continuously undergoes remodeling marked by the change of the ECM structural components, such as COL1A1, COL11A1, fibronectin, and versican.
The matrix-degrading enzyme MMPs such as MMP1, MMP2, and MMP9 are highly involved in this process. Additional players in this process are the LOX family members of lysyl oxidase. They catalyze the first step of the covalent cross-linking of ECM proteins, collagens, and elastin, which contributes to ECM stiffness and mechanical properties.
Relevant Antibodies
| Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|
| AMRe13970 | MMP1 (7R3) Rabbit Monoclonal Antibody | IHC-P,ICC/IF,FC,IF-P | Human |
| AMRe21168 | MMP2 Rabbit Monoclonal antibody | WB,IF,IP,ELISA | Human,Mouse,Rat |
| AMM81024 | MMP9 Mouse Monoclonal Antibody | IHC,ICC,FC,ELISA | Human,Mouse,Rat,Rabbit,Monkey |
| AMRe21559 | MMP13 Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse |
| AMRe21368 | Fibronectin Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse,Rat |
| AMRe21521 | MMP3 Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse,Rat |
| AMRe13374 | LOX (2U4) Rabbit Monoclonal Antibody | WB,IHC-P,ICC/IF,FC,IP,IF-P | Human,Mouse,Rat |
| AMRe84457 | COL11A1 Rabbit Monoclonal | WB | Human |
| AMRe09208 | Collagen I alpha 1 (15S16) Rabbit Monoclonal Antibody | WB,IP | Human,Mouse,Rat |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | Rabbit | ELISA, WB, Dot blot |
| APS0631 | HRP-conjugated Polyclonal Goat Anti-Mouse IgG(H+L) Secondary Antibody | Mouse | ELISA, WB, Dot blot |
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody | Human,Mouse,Rat,Rabbit,Dog,Monkey | WB,ELISA |
Related Products
References
- Theocharis AD, Manou D, Karamanos NK. The extracellular matrix as a multitasking player in disease. FEBS J. 2019 Aug;286(15):2830-2869. Epub 2019 Apr 11. [PMID: 30908868].
- Zollinger AJ, Smith ML. Fibronectin, the extracellular glue. Matrix Biol. 2017 Jul;60-61:27-37. 2016.07.011. Epub 2016 Aug 3. [PMID: 2749634.
- Paolillo M, Schinelli S. Extracellular Matrix Alterations in Metastatic Processes. Int J Mol Sci. 2019 Oct 7;20(19):4947. [PMID: 31591367].
- Bonnans C, Chou J, Werb Z. Remodelling the extracellular matrix in development and disease. Nat Rev Mol Cell Biol. 2014 Dec;15(12):786-801. [PMID: 25415508].
- Amendola PG, Reuten R, Erler JT. Interplay Between LOX Enzymes and Integrins in the Tumor Microenvironment. Cancers (Basel). 2019 May 26;11(5):729. [PMID: 31130685]
 | Flora Flora is a technical support expert at EnkiLife, familiar with immunology and neuroscience, dedicated to providing customers with high-quality product combinations and technical support to help achieve research in neurodegenerative diseases and other neuroscience areas. |