GlycolysisⅡ
Overview
Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis.
The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified.
Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1,6-bisphosphate to 3-phosphoglyceraldehyde.
Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis.
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers.
Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form.
Relevant Antibodies
| Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|
| AMRe04054 | ALDOA Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC-F,IHC-P,ICC/IF |
| AMRe01949 | ENO1/2/3 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB |
| AMRe14909 | NSE (9L10) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,ICC/IF,FC,IP |
| AMRe15915 | PDK1 (9S9) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IP |
| APRab05241 | PFK-2 car (phospho Ser483) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| AMRe03790 | PFKFB3 Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IP |
| AMRe84448 | PGAM1 Rabbit Monoclonal | Human,Mouse,Rat | WB,FC |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | ||
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody |
Related Products
References
- Mediavilla D, Metón I, Baanante IV. Purification and kinetic characterization of 6-phosphofructo-1-kinase from the liver of gilthead sea bream (Sparus aurata). J Biochem. 2008 Aug;144(2):235-44. [PMID: 18483063].
- Castaldo G, Calcagno G, Sibillo R, Cuomo R, Nardone G, Castellano L, Del Vecchio Blanco C, Budillon G, Salvatore F. Quantitative analysis of aldolase A mRNA in liver discriminates between hepatocellular carcinoma and cirrhosis. Clin Chem. 2000 Jul;46(7):901-6. [PMID: 10894831].
- Pancholi V. Multifunctional alpha-enolase: its role in diseases. Cell Mol Life Sci. 2001 Jun;58(7):902-20. [PMID: 11497239].
 | Flora Flora is a technical support expert at EnkiLife, familiar with immunology and neuroscience, dedicated to providing customers with high-quality product combinations and technical support to help achieve research in neurodegenerative diseases and other neuroscience areas. |