| Detection Principle | Calcein-AM: Calcein-AM is a non-fluorescent cell-permeable dye that can freely pass through the cell membrane of living cells. Inside living cells, Calcein-AM is hydrolyzed by intracellular esterases to generate green fluorescent Calcein. Calcein is a water-soluble fluorescent molecule that cannot pass through the cell membrane, so it is retained inside the cell, thereby labeling living cells. The excitation wavelength of Calcein is 495 nm, and the emission wavelength is 515 nm.Propidium Iodide (PI): PI is a nucleic acid dye that cannot penetrate intact cell membranes but can enter cells with damaged or dead cell membranes. After binding to DNA in the nucleus, PI emits red fluorescence, thereby labeling dead cells. The excitation wavelength of PI is 535 nm, and the emission wavelength is 617 nm.By using Calcein-AM and PI simultaneously, live cells (green fluorescence) and dead cells (red fluorescence) can be quickly distinguished, and detection can be performed using fluorescence microscopy, flow cytometry, or fluorescence microplate readers. |
| Detection Method | Fluorescence method, Flow cytometry, Microplate reader |
| Sample Type | Applicable to most eukaryotic mammalian cells, including adherent cells and certain tissues, but not suitable for fungi and yeast |
| Detection Instrument | Fluorescence microscope, Flow cytometer, Microplate reader, and other fluorescence detection systems |
| Storage Temperature | Store at -20°C in the dark |
| Validity Period | 12 months |
| Transportation Conditions | Ice pack |
