RPMI-1640 (ATCC Modification) Complete Medium

RPMI-1640 (ATCC Modification) Complete Medium

Cat. No.: CMC0010
Size:500mL price:$128 Category: Complete Media Tags:

RPMI 1640 medium is named after the Roswell Park Memorial Institute (RPMI), where it was developed, and 1640 is the code name of the medium. RPMI-1640 medium was originally designed for lymphocyte culture, but is now widely used in the culture of various normal cells and cancer cells, especially suspension cells, and is one of the most widely used culture media. This product contains a variety of ingredients such as amino acids, vitamins, and inorganic salts required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product must be used with serum or serum-free supplements.

 

ATCC modified medium contains additional sodium pyruvate and HEPES, increased sugar content, and reduced sodium bicarbonate content.

 

Details

Form

Liquid

Size

500mL

Components

RPMI-1640 (ATCC Modification)(CMB0051)+10% Nutrient+1% Supplement

Concentration

Ready-to-use

Bacteria Detection

Negative

Fungi Detection

Negative

Mycoplasma Detection

Negative

Endotoxin Content(EU/mL)

<3

L-Glutamine

2mM

NaHCO3

1500mg/L

D-Glucose

4500mg/L

Sodium Pyruvate

1mM

HEPES

10mM

Phenol Red

5mg/L

Balanced Salt Solution

Without

Nucleoside

Without

NEAA

Without

D-Galactose

Without

Transportation

2-8°C

Storage

Store at 2-8°C away from light

Shelf Life

6 months

 

Precautions

1. During the entire process, be sure to pay attention to aseptic operation to avoid contamination;

2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;

3. This product is only used for research or further research, not for diagnosis and treatment.

4. It is not suitable for long time storage at room temperature.

5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.

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