p70 S6 Kinase
p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, Ser244) located wtihin a small, carboxy-terminal region of the S6 protein. p70 S6 kinase has been shown to phosphorylate eIF4B at the rapamycin-sensitive site Ser422 in vivo, and a Ser422Ala mutant of eIF4B shows diminished activity in an in vitro translation assay. Phosphorylation of eEF2K by p70 S6 kinase and p90RSK leads to inactivation of eEF2K, facilitating the dephosphorylation of eEF2 and thus promoting translation.
S6K1 is activated by coordinated phosphorylation by mTORC1 and PDK1
Relevent antibodies
| Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|
| APRab05372 | Ribosomal Protein S6 (phospho Ser235/S236) Rabbit Polyclonal Antibody | Human,Mouse,Rat | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| AMRe21083 | RPS6 (Phospho Ser240/Ser244) Rabbit Monoclonal antibody | Human,Mouse,Rat | WB,IHC,IF,IP,ELISA |
| APRab04598 | eIF4B (phospho Ser422) Rabbit Polyclonal Antibody | Human,Mouse | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| APRab04573 | eEF2K (phospho Ser366) Rabbit Polyclonal Antibody | Human,Rat | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| AMRe21491 | S6K1 Rabbit Monoclonal antibody | Human,Mouse,Rat | WB,IHC,IF,IP,ELISA |
| APS0631 | HRP-conjugated Polyclonal Goat Anti-Mouse IgG(H+L) Secondary Antibody | ||
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody |
Related Products
Super-sensitive ECL chemiluminescent reagent
References
- Pullen N, Thomas G. The modular phosphorylation and activation of p70s6k. FEBS Lett. 1997 Jun 23;410(1):78-82.[PMID: 9247127].
- Ferrari S, Bandi HR, Hofsteenge J, Bussian BM, Thomas G. Mitogen-activated 70K S6 kinase. Identification of in vitro 40 S ribosomal S6 phosphorylation sites. J Biol Chem. 1991 Nov 25;266(33):22770-5.[PMID: 1939282].
- Soukar J, Peppas NA, Gaharwar AK. Organelle-Targeting Nanoparticles. Adv Sci (Weinh). 2025 Feb;12(7):e2411720.[PMID: 39806939].
 | Flora Flora is a technical support expert at EnkiLife, familiar with immunology and neuroscience, dedicated to providing customers with high-quality product combinations and technical support to help achieve research in neurodegenerative diseases and other neuroscience areas. |