KEY FEATURES
| Full Name | Major Vault Protein |
|---|---|
| Synonym | LRP; VAULT1; Lung resistance-related protein |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human MVP in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MVP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MVP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MVP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MVP. You can calculate the concentration of Human MVP in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human MVP ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.48 | 0.96 | 4.91 | 0.45 | 1.02 | 5.23 |
| Standard deviation | 0.03 | 0.05 | 0.22 | 0.03 | 0.05 | 0.2 |
| CV(%) | 5.76 | 4.71 | 4.54 | 6.53 | 4.93 | 3.88 |
RECOVERY
The recovery of Human MVP spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-110 | 101 |
| EDTA plasma (n=8) | 89-103 | 94 |
| Cell culture media (n=8) | 84-100 | 91 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human MVP in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with LRP ELISA Kits
| ABCB1 ELISA Kit | publications with LRP and ABCB1 |
| ABCC1 ELISA Kit | publications with LRP and ABCC1 |
| ABCC3 ELISA Kit | publications with LRP and ABCC3 |
| PTEN ELISA Kit | publications with LRP and PTEN |
| STAT1 ELISA Kit | publications with LRP and STAT1 |
| PTPN11 ELISA Kit | publications with LRP and PTPN11 |
| YPEL4 ELISA Kit | publications with LRP and YPEL4 |
Diseases associated with LRP ELISA Kit
| Heart Diseases | publications with LRP and Heart Diseases |
| Brain Diseases | publications with LRP and Brain Diseases |
| Adenocarcinoma | publications with LRP and Adenocarcinoma |
| Disease Models, Animal | publications with LRP and Disease Models, Animal |
| Neoplasm Metastasis | publications with LRP and Neoplasm Metastasis |
| Hypertension | publications with LRP and Hypertension |
| Fibrosis | publications with LRP and Fibrosis |
| Hypertrophy | publications with LRP and Hypertrophy |
| Breast Neoplasms | publications with LRP and Breast Neoplasms |
| Neurobehavioral Manifestations | publications with LRP and Neurobehavioral Manifestations |
Organs/Tissues associated with LRP ELISA Kit
| Heart | publications with LRP and Heart |
| Blood | publications with LRP and Blood |
| Lung | publications with LRP and Lung |
| Vascular | publications with LRP and Vascular |
| Brain | publications with LRP and Brain |
| Muscle | publications with LRP and Muscle |
| Bone | publications with LRP and Bone |
| Connective Tissue | publications with LRP and Connective Tissue |
| Liver | publications with LRP and Liver |
| Bladder | publications with LRP and Bladder |
