Full Name

Nitric Oxide Synthase 3, Endothelial

Synonym

ECNOS;NOS III;E-NOS;

Assay Type

Sandwich

Reactivity

Human

Range

62.5-4000pg/mL

Sensitivity

37.5pg/mL

Sample Type

Serum,plasma and other biological fluids

UNIProt ID

Microplate

96-wells plate breakable into 12 x 8 wells strip  

◤Test Principle：

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.

◤Storage：

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.