Product Introduction
Phosphorus exists in organisms in two forms: inorganic phosphorus (phosphate) and organic phosphorus, and is involved in processes such as energy, nucleic acid metabolism, and protein phosphorylation. Determining the total phosphorus and inorganic phosphorus content in crops helps evaluate the efficiency of phosphorus utilization and provides a scientific basis for precision fertilization.
Features
Wide applicability: Suitable for a variety of sample types, including serum, plasma, tissue samples , etc.
Detection Principle
Inorganic phosphorus reacts with molybdic acid to form phosphomolybdic acid. Under the action of a reducing agent, phosphomolybdic acid is reduced to molybdenum blue, which has a maximum absorption peak at 660 nm. Within a certain range, the absorbance value is proportional to the concentration. When this kit detects tissue samples, the total protein concentration needs to be determined. It is recommended to use the protein concentration determination kit (BCA method) (Cat. No.: BC00006).
Product Composition
Serial Number | Product Name | Packing Specifications ( 100T ) | Storage |
Reagent 1 | Chromogen A | 20 mL | Store at -20℃ , store at 2-8 ℃ after opening |
Reagent 2 | Chromogen B | powder | Store at -20℃ away from light , store at 2-8℃ after opening |
Reagent 3 | Chromogen C | powder | Store at -20℃ away from light , store at 2-8℃ after opening |
Reagent 4 | Protein Precipitants | 40 mL | Store at -20℃ , store at 2-8 ℃ after opening |
Reagent 5 | 2 mmol/L Phosphorus Standard | 1 mL | Store at -20℃ , store at 2-8 ℃ after opening |
96-well ELISA plate | 1 plate | RT | |
96-well membrane | 2 pieces | RT |
Note: The reagents must be stored strictly according to the storage conditions in the table above. Reagents from different test kits cannot be mixed. For reagents with a small volume, centrifuge them before use to avoid insufficient amount of reagent.
Storage conditions
The unopened kit can be stored at -20°C for 6 months.
Notes
This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, shall not be used as food or medicine, and shall not be stored in ordinary residences.
