Product Introduction
Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation.
Detection principle
PDC catalyzes the decarboxylation of pyruvate to produce acetaldehyde. Acetaldehyde reacts under the action of alcohol dehydrogenase (ADH) and catalyzes the conversion of NADH into NAD+. NADH has a characteristic absorption peak at 340nm. The PDC activity can be calculated by measuring the rate of decrease of light absorption at 340nm.
When this kit detects tissue samples and cell samples, the total protein concentration needs to be determined. It is recommended to use the BCA kit for determination. (Cat. No.: BC00006 )
Product Composition
Serial Number | Product Name | Packing Specifications ( 100T ) | Storage |
Reagent 1 | Buffer | 30mL | -20℃, store at 2-8℃ after use. |
Reagent 2
| Substrate A | 1 bottle | -20℃, store at 2-8℃ away from light after use. |
Reagent 3 | Enzyme reagents | 1 piece | Store at 2-8℃ before use and -20℃ after use. |
Reagent 4 | Substrate B | 1 piece | Store at 2-8℃ before use and -20℃ away from light after use. |
Consumables 1 | 96 -well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage Conditions
After receiving the test kit, please store it according to "Product Components-Storage Method". The validity period is 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.
