Product Introduction
Glucose oxidase is an important industrial enzyme in the food industry. It is widely used in deoxygenation of foods such as wine, beer, juice, and milk powder, flour improvement, and prevention of food browning. It is also widely used in rapid food detection and biosensors. Microorganisms grow and reproduce quickly and have a wide range of sources. They are the main source of GOD production. The main production strains are Aspergillus niger and Penicillium.
Product Features
This kit uses glucose oxidase as the standard instead of unstable hydrogen peroxide, which makes the kit have higher accuracy and better stability.
Principle
Glucose generates hydrogen peroxide under the action of glucose oxidase, and hydrogen peroxide causes the chromogen to develop color under the action of peroxidase. The activity of GOD is determined by the absorbance at 550nm.
When this kit is used to detect samples, the total protein concentration needs to be determined. The BCA method (Cat. No.: BC00006) is recommended. The figure below shows the standard curve determined by this kit.
The following standard curve is for reference only:
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Buffer | 30ml × 1 vial | -20℃ |
Reagent 2 | Enzyme Reagent | Powder × 1 vial | -20℃ |
Reagent 3 | Color Developer | 1ml x 2 vials | -20℃ |
Reagent 4 | Standard | 1ml x 1 vial | -20℃ |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20°C for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
