Product Introduction
Aspartate aminotransferase (AST), also known as aspartate aminotransferase (GOT), is an enzyme widely found in animals, plants, microorganisms and cultured cells. It catalyzes the transamination reaction between aspartate and α-ketoglutarate, which is crucial in amino acid metabolism. Clinically, AST/GOT is often used as an auxiliary diagnostic indicator for myocardial infarction and myocarditis. At the same time, its concentration in serum will also increase when the liver is damaged.
Detection principle
AST/GOT can transfer the amino and keto groups of ketoglutaric acid and aspartic acid to generate glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate to pyruvic acid during the reaction. Pyruvic acid reacts with 2,4-dinitrophenylhydrazine to generate 2,4-dinitrophenylhydrazone, which is reddish brown in alkaline solution. After colorimetry, the enzyme activity unit can be obtained by checking the standard curve.
Product Composition
Serial Number | Product Name | Packing Specifications (100T) | Storage |
Reagent 1 | Substrate matrix liquid | 5 mL | 2-8 ℃ after opening . |
Reagent 2
| Color developer | 5 mL | 2-8 ℃ away from light after opening . |
Reagent 3 | Alkaline solution | 5 mL | 2-8 ℃ after opening . |
Reagent 4 | 2 μmol /mL Sodium Pyruvate Standard | 0.5 mL | 2-8 ℃ after opening . |
Reagent 5 | Buffer | 0.5 mL | 2-8 ℃ after opening . |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage Conditions
The unopened test kit can be stored at -20 ℃ for 12 months, and after opening, it can be stored at 2-8 ℃ for 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.
