Product Introduction
Triglycerides are widely present in nature and are usually stored in animals and plants in a non-hydrated form. They are one of the main forms of energy acquisition and storage for animals and humans, and are also the main component of fat in food.
Principle
Triglycerides (TG) can be hydrolyzed into glycerol and free fatty acids by lipoprotein lipase. Glycerol generates glycerol-3-phosphate and ADP under the action of glycerol kinase (GK). Glycerol-3-phosphate generates hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to generate red quinone compounds of benzoquinone imine phenazone, and its color depth is proportional to the TG content (the principle diagram is as follows).
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Enzyme Working Solution | 10 mL | -20℃, store at 2-8°C in the dark after opening. |
Reagent 2 | 10 mmol/L Standard | 0.1 mL | -20℃, store at 2-8°C after opening. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months, and after opening, it can be stored at 2-8℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
