Product Introduction
Total cholesterol refers to the sum of cholesterol contained in all lipoproteins in the blood. The total cholesterol level of a population mainly depends on genetic factors and lifestyle. Total cholesterol includes free cholesterol and cholesterol esters, and the liver is the main organ for synthesis and storage.
Principle
Total cholesterol (TC) includes free cholesterol and cholesterol esters. Cholesterol esters can be hydrolyzed into cholesterol and free fatty acids by cholesterol esterase (CE). Cholesterol generates △4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase (CO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to generate red quinone compounds of benzoquinone imine phenazone. The color depth is proportional to the TC content. There is a special absorption peak at 510nm. Based on this, the total cholesterol content in the sample can be calculated from the absorbance value. The detection principle is shown in the figure below:
The following standard curve is for reference only:
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Enzyme Working Solution | 30mL | -20℃, store at 2-8°C after opening. |
Reagent 2 | 10mmol/L Cholesterol Standard Solution | 0.2mL | -20℃, store at 2-8°C after opening. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months, and after opening, it can be stored at 2-8℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
