Product Introduction
In organisms, proline is not only an ideal osmotic regulating substance, but also can be used as a protective substance for membranes and enzymes and a free radical scavenger, thereby protecting the growth of plants under osmotic stress. For the accumulation of potassium ions, another important osmotic regulating substance in the body, in the vacuole, proline can also regulate the osmotic balance of the cytoplasm.
Principle
Among the amino acids in plants, only proline (Pro) can react with acidic ninhydrin to generate a stable red product (as shown below). This product has a maximum absorption peak at a wavelength of 520 nm, and its absorption value is linearly related to the proline content. Therefore, the proline content in the sample can be determined by the acidic ninhydrin method.
The following standard curve is for reference only:
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | Extraction buffer | 50 mLx1 bottle | -20℃, store at 2-8°C away from light after opening. |
Reagent 2 | Ninhydrin | 1.5 g x1 bottle | -20℃, store at 2-8°C away from light after opening. |
Reagent 3 | Acid reagent | 20 ml x1 bottle | -20℃, store at 2-8°C away from light after opening. |
Reagent 4 | 400 μg/mL standard | 1 ml x1 tube | -20℃, store at 2-8°C away from light after opening. |
Consumable 1 | Microplate(96 wells) | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened test kit can be stored at -20℃ for 12 months. After opening, it can be stored at 2-8℃ away from light for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
