Product Introduction
Active thiol (SH) mainly exists in cysteine, which is an important group in protein structure and certain redox reactions in organisms. The disulfide bond formed by the dehydrogenation of two thiol groups in the protein structure allows adjacent polypeptides to be connected. It is very important for maintaining the integrity of the protein structure. All animal tissues contain different amounts of active thiol groups. Glutathione, which plays an important role in the body, mainly relies on the reduction effect of the active thiol groups in the cysteine it contains. Thiol groups are also active groups of many enzymes. Some heavy metal salts such as Hg2+ bind to enzyme thiol groups and affect enzyme activity. Thiol compounds are closely related to many functional activities of the body, the effects of drugs and poisons, and the occurrence and development of certain diseases. The determination of thiol content in tissues and blood has been increasingly valued by people in order to explore the research significance in the occurrence mechanism, treatment, prevention and prognosis of certain diseases.
Detection Principle
The mercapto compounds react with 5,5'-dithiobis(2-nitrobenzoic acid) to generate a yellow product under neutral or alkaline conditions with a maximum absorption peak at 412 nm.
The figure below shows the standard curve for the determination of total thiol groups by this kit . The following standard curve is for reference only:
Product composition
Serial number | Product Name | Packing Specifications ( 100T ) | Storage |
Reagent 1 | Buffer | 20 mL | store at 2-8℃ after opening |
Reagent 2 | Color developer | 1.3 mL | store at 2-8℃ after opening |
Reagent 3 | Total Thiol Standards | powder | store at 2-8℃ after opening |
Reagent 4 | Dissolving agent | 20 ml | store at 2-8℃ after opening |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage conditions
The unopened kit can be stored at -20℃ for 12 months.
After opening the bottle, it can be stored at 2-8 °C and is valid for 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.
