Product Introduction
Detecting the activity of catalase in cells, tissues, or other samples through a colorimetric reaction. Catalase is a common enzyme that plays an important role in redox reactions within organisms and is widely distributed in the liver, kidneys, and red blood cells.
Principle
Under conditions where hydrogen peroxide is relatively abundant, catalase can catalyze the decomposition of hydrogen peroxide to produce water and oxygen. Unreacted residual hydrogen peroxide can oxidize a colorimetric substrate under the catalysis of peroxidase, resulting in a red product with a maximum absorption wavelength of 520 nm. By measuring A520 and comparing it with a standard curve made from hydrogen peroxide standards, the amount of hydrogen peroxide consumed by catalase in the sample can be calculated, and thus the enzyme activity of catalase in the sample can be determined.
The figure below shows the A520 readings for hydrogen peroxide standards detected by this kit.
Components
Components | Size (100T) | Storage |
Catalase Assay Buffer | 60 ml | -20℃, store at 2-8℃ after opening. |
Hydrogen Peroxide (approximately 1 M) | 5 ml | -20℃ |
Catalase Reaction Stop Solution | 50 ml | -20℃, store at 2-8℃ after opening. |
Chromogenic Substrate | 20 ml | -20℃ |
Peroxidase | 20 μl | -20℃ |
Microplate | 1 plate | RT |
Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20°C for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
