Product Introduction
Lactic acid plays a role in energy metabolism, pH regulation, and signal transmission in the human body. Lactic acid is effectively cleared by the body under normal circumstances, but in some cases, lactate levels may increase, leading to lactic acidosis. Lactic acidosis can be caused by a variety of reasons, including hypoxia, liver dysfunction, metabolic disorders, etc. In clinical practice, the degree of lactic acidosis can be assessed and treatment can be guided by measuring the lactate concentration in the blood.
Product Features
Easy to use, high sensitivity and wide linear range.
Principle
With oxidized coenzyme I (NAD+) as hydrogen acceptor, lactate dehydrogenase (LDH) catalyzes the dehydrogenation of lactate to produce pyruvate, converting NAD+ into reduced coenzyme I (NADH). Among them, N-methylphenazine methylsulfate (PMS) transfers hydrogen to reduce WST-8 to an orange coloring substance, the absorbance of which produces an absorption peak at a wavelength of 450nm, and the absorbance is linearly related to the lactic acid content.
The figure below shows the standard curve of D-lactic acid determination by this kit:
Components
No. | Components | Size (50T) | Size (100T) | Storage |
Reagent 1 | Buffer | 10 mL | 20 mL | -20℃, store at 2-8℃ after opening. |
Reagent 2 | Enzyme Stock Solution | 0.06 mL | 0.12 mL | -20℃, store at 2-8℃ after opening. |
Reagent 3 | Color Developer | 1.2 mL | 1.2 mL/vial, 2 vials | -20℃, protect from light, store at 2-8℃ after opening. |
Reagent 4 | Substrate B | 12 mL | 24 mL | -20℃, store at 2-8℃ after opening. |
Reagent 5 | 10 mmol/L Standard | 1 mL | 1 mL/vial, 2 vials | -20℃, store at 2-8℃ after opening. |
Consumable 1 | Microplate | 1 plate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months, and after opening, it can be stored at 2-8℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
