KEY FEATURES
| Full Name | Transforming Growth Factor Beta Induced Protein |
|---|---|
| Synonym | TGF-BI; CSD3; LCD1; CSD1; CSD2; BIGH3; CDB1; CDGG1; RGD-CAP; Kerato-Epithelin; RGD-Containing Collagen-Associated Protein |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 62.5-4000pg/mL |
| Sensitivity | 24.1pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse TGFbI in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGFbI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGFbI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGFbI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TGFbI. You can calculate the concentration of Mouse TGFbI in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse TGFbI ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 190.74 | 346.32 | 1493.95 | 181.06 | 316.11 | 1536.43 |
| Standard deviation | 10.51 | 16.07 | 50.2 | 11.77 | 13.69 | 83.74 |
| CV(%) | 5.51 | 4.64 | 3.36 | 6.5 | 4.33 | 5.45 |
RECOVERY
The recovery of Mouse TGFbI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-108 | 99 |
| EDTA plasma (n=8) | 89-103 | 95 |
| Cell culture media (n=8) | 94-108 | 100 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse TGFbI in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Diseases associated with Tgfbi ELISA Kit
| Adenocarcinoma | publications with Tgfbi and Adenocarcinoma |
| Corneal dystrophy Avellino type | publications with Tgfbi and Corneal dystrophy Avellino type |
| Lattice corneal dystrophy type 1 | publications with Tgfbi and Lattice corneal dystrophy type 1 |
| Neoplasm Metastasis | publications with Tgfbi and Neoplasm Metastasis |
| Lung Diseases | publications with Tgfbi and Lung Diseases |
| Inflammation | publications with Tgfbi and Inflammation |
| Ovarian Neoplasms | publications with Tgfbi and Ovarian Neoplasms |
| Kidney Diseases | publications with Tgfbi and Kidney Diseases |
| Lung Neoplasms | publications with Tgfbi and Lung Neoplasms |
| Fibrosis | publications with Tgfbi and Fibrosis |
Organs/Tissues associated with Tgfbi ELISA Kit
| Eye | publications with Tgfbi and Eye |
| Blood | publications with Tgfbi and Blood |
| Bone | publications with Tgfbi and Bone |
| Lung | publications with Tgfbi and Lung |
| Brain | publications with Tgfbi and Brain |
| Kidney | publications with Tgfbi and Kidney |
| Liver | publications with Tgfbi and Liver |
| Muscle | publications with Tgfbi and Muscle |
| Bone Marrow | publications with Tgfbi and Bone Marrow |
| Connective Tissue | publications with Tgfbi and Connective Tissue |
