KEY FEATURES
| Full Name | Ataxia Telangiectasia Mutated |
|---|---|
| Synonym | AT1; ATD; ATA; ATC; ATDC; ATE; TEL1; TELO1; Serine-protein kinase ATM |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6.4pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ATM in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ATM. You can calculate the concentration of Human ATM in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ATM ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 52.9 | 128.43 | 392.32 | 58.13 | 120.74 | 369.02 |
| Standard deviation | 3.63 | 5.55 | 15.22 | 3.81 | 6.83 | 15.43 |
| CV(%) | 6.87 | 4.32 | 3.88 | 6.56 | 5.66 | 4.18 |
RECOVERY
The recovery of Human ATM spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-102 | 94 |
| EDTA plasma (n=8) | 93-105 | 99 |
| Cell culture media (n=8) | 95-111 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ATM in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ATM ELISA Kits
| BRCA1 ELISA Kit | publications with ATM and BRCA1 |
| CHEK2 ELISA Kit | publications with ATM and CHEK2 |
| TP53 ELISA Kit | publications with ATM and TP53 |
| H2AFX ELISA Kit | publications with ATM and H2AFX |
| MRE11A ELISA Kit | publications with ATM and MRE11A |
| TP53BP1 ELISA Kit | publications with ATM and TP53BP1 |
| RAD17 ELISA Kit | publications with ATM and RAD17 |
| KAT5 ELISA Kit | publications with ATM and KAT5 |
| CHEK1 ELISA Kit | publications with ATM and CHEK1 |
| ABL1 ELISA Kit | publications with ATM and ABL1 |
Pathways associated with ATM ELISA Kit
| ATM Mediated Phosphorylation Of Repair Proteins Pathway | ATM Mediated Phosphorylation Of Repair Proteins Pathway |
| ATM Mediated Response To DNA Double-strand Break Pathway | ATM Mediated Response To DNA Double-strand Break Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
| Autodegradation Of The E3 Ubiquitin Ligase COP1 Pathway | Autodegradation Of The E3 Ubiquitin Ligase COP1 Pathway |
| BARD1 Signaling Events Pathway | BARD1 Signaling Events Pathway |
| BRCA1-associated Genome Surveillance Complex (BASC) Pathway | BRCA1-associated Genome Surveillance Complex (BASC) Pathway |
| Canonical NF-kappaB Pathway | Canonical NF-kappaB Pathway |
| Cell Cycle Pathway | Cell Cycle Pathway |
Diseases associated with ATM ELISA Kit
| Ataxia Telangiectasia | publications with ATM and Ataxia Telangiectasia |
| Nervous System Diseases | publications with ATM and Nervous System Diseases |
| Cardiovascular Diseases | publications with ATM and Cardiovascular Diseases |
| Brain Diseases | publications with ATM and Brain Diseases |
| Breast Neoplasms | publications with ATM and Breast Neoplasms |
| Disease Models, Animal | publications with ATM and Disease Models, Animal |
| Heart Diseases | publications with ATM and Heart Diseases |
| Lung Diseases | publications with ATM and Lung Diseases |
| Inflammation | publications with ATM and Inflammation |
| Adenocarcinoma | publications with ATM and Adenocarcinoma |
Organs/Tissues associated with ATM ELISA Kit
| Blood | publications with ATM and Blood |
| Brain | publications with ATM and Brain |
| Lung | publications with ATM and Lung |
| Vascular | publications with ATM and Vascular |
| Heart | publications with ATM and Heart |
| Muscle | publications with ATM and Muscle |
| Liver | publications with ATM and Liver |
| Skin | publications with ATM and Skin |
| Kidney | publications with ATM and Kidney |
| Nerve | publications with ATM and Nerve |
