BAF Complex
Introduction
ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair. The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin.
The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes. BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines.
SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/BAF47 are members of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro. ARID1A/BAF250A is one of the accessory subunits of the SWI/SNF complex. SMARCC1, SMARCB1, and ARID1A are an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF). SMARCC1 is necessary for early embryogenesis, especially proper brain and visceral endoderm development. SMARCB1 is necessary for early embryogenesis and hepatocyte differentiation. ARID1A is critical for ES cell pluripotency and differentiation into mesoderm-derived cardiomyocytes and adipocytes.
While SMARCC2 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2 leads to loss of stem cell pluripotency and self renewal.
Relevant Antibodies
| Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|
| AMRe83877 | ARID1A Rabbit Monoclonal | Human,Mouse,Rat | WB,IHC,ICC,IF |
| AMRe21379 | BRG1 Rabbit Monoclonal antibody | Human,Rat | WB,IHC,IF,IP,ELISA |
| AMRe03206 | SMARCA2 Rabbit Monoclonal Antibody | Human | WB,IHC-F,IHC-P,ICC/IF |
| AMRe18053 | SNF5 (19H18) Rabbit Monoclonal Antibody | Human,Mouse,Rat | WB,IHC-P,ICC/IF,FC,IP,IF-P |
| AMRe18006 | SMARCC1/BAF155 (11P17) Rabbit Monoclonal Antibody | Human,Rat | WB,ICC/IF,FC,IP |
| APRab07429 | BAF170 Rabbit Polyclonal Antibody | Human,Mouse | WB,IF-P,IF-F,ICC/IF,ELISA |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | Rabbit | ELISA, WB, Dot blot |
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody | Human,Mouse,Rat,Rabbit,Dog,Monkey | WB,ELISA |
| AMRe83877 | ARID1A Rabbit Monoclonal | Human,Mouse,Rat | WB,IHC,ICC,IF |
| AMRe21379 | BRG1 Rabbit Monoclonal antibody | Human,Rat | WB,IHC,IF,IP,ELISA |
Related Products
References
- Ho L, Crabtree GR. Chromatin remodelling during development. Nature. 2010 Jan 28;463(7280):474-84. [PMID: 20110991].
- Becker PB, Hörz W. ATP-dependent nucleosome remodeling. Annu Rev Biochem. 2002;71:247-73. Epub 2001 Nov 9. [PMID: 12045097].
- Lessard JA, Crabtree GR. Chromatin regulatory mechanisms in pluripotency. Annu Rev Cell Dev Biol. 2010;26:503-32. [PMID: 20624054].
- Yamamichi N, Yamamichi-Nishina M, Mizutani T, et,al. The Brm gene suppressed at the post-transcriptional level in various human cell lines is inducible by transient HDAC inhibitor treatment, which exhibits antioncogenic potential. Oncogene. 2005 Aug 18;24(35):5471-81. [PMID: 16007216].
- Shen H, Powers N, Saini N,et,al. The SWI/SNF ATPase Brm is a gatekeeper of proliferative control in prostate cancer. Cancer Res. 2008 Dec 15;68(24):10154-62. [PMID: 19074882].
- Schaniel C, Ang YS, Ratnakumar K, et,al. Smarcc1/Baf155 couples self-renewal gene repression with changes in chromatin structure in mouse embryonic stem cells. Stem Cells. 2009 Dec;27(12):2979-91. [PMID: 19785031].
- Ho L, Ronan JL, Wu J, Staahl BT, et,al. An embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5181-6. doi: 10.1073/pnas.0812889106. Epub 2009 Mar 11. PMID: 19279220; PMCID: PMC2654396.
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