Custom Monoclonal Antibody Development

EnkiLife | Custom Monoclonal Antibody Development Services

Custom Monoclonal Antibody Development

High-specificity monoclonal antibodies.

End-to-end mAb development: from antigen design and immunization to hybridoma development, subcloning, and application-specific screening. Ideal for WB, IHC, IP, FC, and ELISA. Guaranteed positive clones and fast turnaround.

View Packages                Key Features
Monoclonal Antibody

Why Choose Monoclonal Antibodies?

  • Unparalleled Specificity – Recognizes a single epitope, eliminating cross-reactivity and ensuring precise targeting in complex assays like IHC, FC, and ELISA.
  • Consistent & Reproducible – Infinite supply of identical antibodies provides batch-to-batch uniformity, critical for long-term studies and diagnostic applications.
  • High Sensitivity with Low Background – Clonal selection yields high-affinity binders with minimal non-specific binding, ideal for detecting low-abundance targets.
  • Custom Design & Screening – Development includes functional screening (e.g., neutralizing, phosphorylation-specific) to match exact application needs.
  • Scalable Production – Hybridoma or recombinant platforms enable stable, large-scale manufacturing with defined genetic sequences.
Monoclonal Antibody

Monoclonal Antibody Development Packages

Flexible packages for mouse, rabbit, and rat hosts. All include hybridoma development, subcloning, and QC.

Monoclonal Workflow
MilestoneActivities & SpecificationsDeliverablesTimeline
Antigen Preparation (Optional)
Protein: customer provided or EnkiLife expressed
Peptide: customer provides or EnkiLife synthesized
Remaining unused antigen delivered at project completion1-2 weeks
(Protein expression: project dependent)
Immunization, Fusion & ScreeningImmunization (4-6 weeks), fusion, HAT selection, ELISA screening20+ ELISA-positive supernatants6-10 weeks
Subcloning & Cryopreservation2 rounds of limited dilution subcloning, expansion, freezing3 monoclonal hybridoma lines per target5-8 weeks
Purification & QCSmall-scale production, protein A/G purification, QC testingPurified antibody (0.1-1 mg), CoA2-3 weeks

Immunogen Requirements (customer-provided protein)

  • Purity ≥ 75%
  • Quantity ≥ 1.5 mg
  • Concentration ≥ 0.4 mg/ml
  • Homology ≤ 90%
  • MW >10 KDa
  • Tags His/Flag accepted

⚬ For additional customization (e.g., larger scale, sequencing) please inquire.

Additional & Related Services

Explore our complete antibody platform and technical guides.

Small Molecule

Small Molecule & PTM Antibody Development

Targeted small molecule/phosphorylation-state screening,PTM antibody counter-screening.

Learn More
Anti-idiotype

Anti-idiotype Antibody Services

For PK/PD and immunogenicity testing. Developed against your therapeutic antibody.

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Single B Cell

Rabbit Single B Cell Platform

Directly clone rabbit IgG sequences from single B cells,expression in CHO/HEK293 systems, bypassing hybridoma fusion.

Learn More
Engineering

Sequencing & Engineering Antibody

Sequencing, cloning, and expression in CHO/HEK293. Humanization, affinity maturation, bispecific design.

Learn More

Simple Quotation & Ordering

1

Submit Request

Email order@enkilife.com with target and host preference.

2

Consultation

TAM contacts you within 24h for strategy and quote.

3

Approval

Review and approve final order details and price.

4

Project Start

Project initiated; PM provides updates until delivery.

Start Your Monoclonal Project

To discuss your target and receive a customized proposal, please email order@enkilife.com. Our team is ready to assist you right away.

⬇ Download Antibody Request Form
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