Total Iron Assay Kit

Total Iron Assay Kit

Product Introduction

Iron is one of the essential trace elements for the human body and is crucial for physiological processes such as oxygen transport and fat oxidation. The determination of iron ion content is of great significance for evaluating nutritional status, diagnosing iron metabolism-related diseases, and studying the role of iron in biological processes.


Product Features

This kit is convenient and quick. The absorbance measurement can be carried out 3-5 minutes after adding the sample to be tested. Usually 10-20 samples can be tested in more than ten minutes.


Principle

Under the action of the reducing agent, the iron ions in the sample are reduced to ferrous ions. Fe2+ forms a blue complex with tripyridyl triazine under acidic conditions, with an absorption peak at 593nm. The content of Fe2+ can be calculated by measuring the absorbance at this wavelength.


Components

No.

Components

Size (100T)

Storage

Reagent 1

Detection Buffer

20 mL

-20°C, protected from light, store at 2-8°C after opening.

Reagent 2

Color Development Solution

6 mL

-20°C, protected from light, store at 2-8°C after opening.

Reagent 3

Reducing Agent

6 mL

-20°C, protected from light, store at 2-8°C after opening.

Reagent 4

10 mmol/L Iron Ion Standard

1 mL/vial, 2 vials

-20°C, protected from light, store at 2-8°C after opening.

Consumable 1

Microplate

1 plate

RT

Consumable 2

Plate Sealer

2 pieces

RT


Storage

The unopened kit can be stored at -20 for 12 months. After opening, it can be stored at 2-8 for 6 months.


Notes

This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.