Product Introduction
Iron is one of the essential trace elements for the human body and is crucial for physiological processes such as oxygen transport and fat oxidation. The determination of iron ion content is of great significance for evaluating nutritional status, diagnosing iron metabolism-related diseases, and studying the role of iron in biological processes.
Product Features
This kit is convenient and quick. The absorbance measurement can be carried out 3-5 minutes after adding the sample to be tested. Usually 10-20 samples can be tested in more than ten minutes.
Principle
Based on the coordination effect between tetrasodium dimethylol orange and Fe3+. When tetrasodium dimethylol orange is added to a solution containing Fe3+, it can form a stable orange-red complex with Fe3+. It can be used to quantitatively detect the concentration of trivalent iron ions. In the absence of Fe3+, tetrasodium dimethylol orange is usually yellow or orange; in the presence of Fe3+, the color of the solution will turn to red. There is an absorption peak at 560nm, and the content of Fe3+ can be calculated by measuring the absorbance at this wavelength.
Fe3++3XO→Fe(XO)3
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Detection Buffer | 20 mL | -20°C, protected from light, store at 2-8°C after opening. |
Reagent 2 | Color Development Solution | 15 mL | -20°C, protected from light, store at 2-8°C after opening. |
Reagent 3 | 10 mmol/L Iron Ion Standard | 2 mL | -20°C, protected from light, store at 2-8°C after opening. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months. After opening, it can be stored at 2-8 ℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
