Product Introduction
Lipid oxidation refers to the process in which lipid molecules in cells or the body suffer oxidative damage, which can lead to impaired cell and tissue function and cause a series of diseases. In medical and life science research, one of the methods to measure the degree of lipid oxidation is to detect the level of lipid oxidation product malondialdehyde (MDA).
Product Features
This kit can measure MDA in the range of 1-200 μM. The figure below shows the A532 readings of different concentrations of MDA standards.
Principle
This kit uses thiobarbituric acid (TBA) to react with MDA to produce a red product (MDA-TBA adduct). The MDA-TBA adduct has a maximum absorption at 532 nm,
which can be detected by colorimetry.In addition, the MDA-TBA adduct can also be excited at 532nm to produce a maximum emission wavelength of 532nm,
which can also be used for fluorescence detection.
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | TBA | 25 mg | -20℃, keep away from light, after opening the bottle can be stored at room temperature or 2-8 degrees for 3 months. |
Reagent 2 | TBA preparation solution | 6.76 ml | -20℃, after opening the bottle can be stored at room temperature or 2-8 degrees for 3 months. |
Reagent 3 | TBA diluent | 15 ml | -20℃, after opening the bottle can be stored at room temperature or 2-8 degrees for 3 months. |
Reagent 4 | Antioxidants | 300 μl | -20℃, keep away from light. |
Reagent 5 | Standard (1 mM ) | 200 μl | -20℃ |
Consumable 1 | Microplate(96 wells) | 1 board | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
