Product Introduction
Pyruvate is an important intermediate in the sugar metabolism of all biological cells and the mutual transformation of various substances in the body. Because the molecule contains activated ketone and carboxyl groups, it is widely used as a basic chemical raw material in various fields such as chemistry, pharmaceuticals, food, agriculture and environmental protection.
Principle
Pyruvic acid reacts with a color developer, and the reaction product is reddish brown in an alkaline solution. The depth of the color is proportional to the pyruvic acid content, and the pyruvic acid content can be calculated by colorimetry. When this kit detects tissue samples, the total protein concentration needs to be determined. It is recommended to use the protein concentration determination kit (BCA method) (BC00006) produced by EnkiLife.
The figure below shows the standard curve of pyruvate determination by this kit:
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Clarifying Agent | 0.6 mL | -20℃, store at 2-8℃ after opening. |
Reagent 2 | Color Developer | 3 mL | -20℃, protect from light, store at 2-8℃ after opening. |
Reagent 3 | Alkaline Reagent | 10 mL | -20℃, store at 2-8℃ after opening. |
Reagent 4 | 2 μmol/mL Sodium Pyruvate Standard | 0.8 `mL/vial, 2 vials | -20℃, store at 2-8℃ after opening. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months, and after opening, it can be stored at 2-8℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
