Product List

APPBP1 Rabbit Monoclonal Antibody

Size:50μL Price:$158
Size:100μL Price:$288
Size:200μL Price:$520
Application:WB,IP
Reactivity:Human
Conjugate:Unconjugated
Optional conjugates: Biotin, FITC (free of charge). See other 26 conjugates.
Gene Name:NAE1

SKU: AMRe01665

Detail

Summary

Production Name	APPBP1 Rabbit Monoclonal Antibody
Description	Recombinant rabbit monoclonal antibody
Host	Rabbit
Application	WB,IP
Reactivity	Human

Performance

Conjugation	Unconjugated
Modification	Unmodified
Isotype	IgG
Clonality	Monoclonal
Form	Liquid
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Buffer	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification	Affinity Purification

Immunogen

Gene Name	NAE1
Alternative Names	NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1
Gene ID	8883
SwissProt ID	Q13564

Application

Dilution Ratio	WB 1:500-1:1000,IP 1:20-1:50
Molecular Weight	Calculated MW: 60 kDa; Observed MW: 60 kDa

Background

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Research Area

Cell Biology