Size1：20mL Price1：$80
Size2：50mL Price2：$150
Size3：100mL Price2：$240
Product Information
Obtaining intact protein samples from cells or tissues is one of the critical factors influencing the authenticity and reliability of Western blotting (WB) results. This product is a revolutionary solution specifically designed to extract complete protein samples, overcoming the limitations of traditional RIPA lysis buffers and ultrasonic disruption techniques. It enables rapid and complete extraction of proteins from cells and tissues while effectively protecting protein chemical modifications. It is particularly suitable for the extraction of large molecular weight proteins and post-translationally modified proteins, ensuring high-quality samples for protein analysis experiments such as Western blotting.
Components
Components | Size(20ml) | Size(50ml) | Size(100ml) |
Reagent A | 40 μL | 100 μL | 200 μL |
Reagent B | 20 mL | 50 mL | 100 mL |
Key Features
l Complete extraction of large molecular weight proteins.
l Protection of post-translational modifications (e.g., phosphorylation, glycosylation, ubiquitination, methylation, and acetylation).
l One-step process without requiring additional enzyme inhibitors.
l Compatible with both cell and tissue samples.
Validation Data
• Comparative Experiment on Large Molecular Weight Protein Extraction
Figure A: HeLa cells lysed with RC0005 and RIPA buffer were analyzed by Western blot using mTOR (289 kDa) antibody. Lysate loading: 50 μg. Results show higher abundance of large molecular weight proteins in RC0005-treated samples.
Figure B: HT1080 cells lysed with RC0005 (30 min, 15 min, 5 min) or ultrasonic disruption were analyzed by Western blot using SMRT antibody (270/160 kDa). Lysate loading: 50 μg. Ultrasonic treatment fragmented SMRT into smaller bands, while RC0005 preserved protein integrity across all time points.
• Comparative Experiment on Protein Post-Translational Modifications
HeLa cells lysed with RC0005 and RIPA were analyzed by Western blot using O-GlcNAc (glycosylation) antibody (parallel groups). Lysate loading: 50 μg. RC0005-treated samples exhibited significantly higher abundance of glycosylated proteins.
• Sample Compatibility Experiment
Figure A: Western blot analysis of HeLa cells and mouse brain tissue lysed with RC0005 using mTOR (289 kDa) antibody. Lysate loading: 50 μg.
Figure B: Western blot analysis of the same samples using GAPDH (37 kDa) antibody.
Data demonstrate RC0005’s excellent lysis efficiency for both cell and tissue samples, with compatibility across proteins of varying sizes.
Storage Conditions
Component A: Store at -20°C.
Component B: Store at room temperature or 4°C.
Note: Prolonged storage of Component B at 4°C may cause precipitation, which does not affect product quality. The precipitate will dissolve upon returning to room temperature.
Applications
Extraction of large molecular weight proteins；Extraction of post-translationally modified proteins (e.g., glycosylated, phosphorylated, ubiquitinated)；Western blot analysis.