Product List

2-Hour Rapid Western Blot Ready-to-Use Complete Workflow Kit

Size：2T Price：$150

SKU: RA10020

Detail

EnkiLife 2-Hour Rapid Western Blot Ready-to-Use Complete Workflow Kit | Highly Efficient & Stable

Product Overview

Western Blot (WB), as a core immunological experiment, often presents challenges due to complex workflows and inconsistent results. EnkiLife's new 2-Hour Rapid WB Ready-to-Use Complete Workflow Kit not only reduces experimental time to just 2 hours but also ensures highly reproducible results through innovative design.

This kit features standardized pre-cast gels and optimized formulations to eliminate batch variations and ensure consistency between experiments. It includes most reagents and consumables required for the complete workflow—users only need to supply water, ethanol/methanol, common antibodies, and related equipment.

Product Features

Ultra-Fast

Complete Western Blot workflow in approximately 2 hours, 5-10x faster than traditional methods

Optimized Workflow

Integrated solution from sample processing to detection—no additional reagents needed

Reliable Results

Excellent reproducibility with batch-to-batch consistency (CV <5%), minimal inter-experiment variation

Rapid Incubation

Proprietary blocking buffer and antibody diluent: 10-min blocking, 15-min primary antibody, 5-min secondary antibody

Ready-to-Use

Pre-cast gels, ready-to-use markers, pre-formulated buffers—use directly out of the box

Lab Safety

Pre-cast gels and odorless buffers prevent exposure to toxic reagents like acrylamide

Exceptional Reproducibility & Consistency

High Batch Consistency

Strict QC ensures consistent performance across batches (CV <5%)

Uniform Band Quality

Optimized pre-cast gel technology minimizes edge effects for sharper bands

Standardized Protocol

Simplified procedures reduce human error and improve reliability

Rigorous QC Standards

Every batch validated through Western Blot performance testing

Reproducibility Comparison: Band Intensity CV

Traditional WB

15-25%

EnkiLife 2-Hour Kit

3-5%

Safety Features

Pre-Cast Gels

Safe polymerization technology eliminates exposure to neurotoxic reagents like acrylamide/TEMED

Odorless Buffers

Sample loading buffer excludes irritants like β-mercaptoethanol for improved lab air quality

Toxic Reagent Avoidance

Full workflow design prevents contact with hazardous chemicals common in traditional WB

Health Protection

Reduces researcher exposure risks and creates a safer lab environment

Traditional WB Duration

1-2 Days

Long waiting times, low efficiency

2-Hour Rapid WB Kit

2 Hours

Complete experiments efficiently

Kit Components

Component	Size 2T(For 30 Assays)
Protein Loading Buffer (5X)	0.2ml
Pre-stained Marker (10-180kDa)	20μl
Pre-Cast Gel (Bis-Tris, 4-20%, 15 wells)	2 units
Electrophoresis Buffer Granules	2 packs (500ml/pack)
PVDF Membrane (0.45μm)	2 sheets
Transfer Sponges (Filter-Free)	4 pieces
Rapid Transfer Buffer Granules	2 pack (1L/pack)
Protein-Free Rapid Blocking Buffer	20ml
Rapid Antibody Diluent	16ml
Rapid Wash Buffer (10X)	14ml
ECL Substrate Solution A	1.2ml
ECL Substrate Solution B	1.2ml

Storage Instructions:

Protein loading buffer and pre-stained marker: -20°C; other reagents: 4°C. Shelf life: 1 year. Full kit at 4°C: minimum 3 months stability.

Consistency Guarantee: All components pass strict QC with batch-to-batch CV <5%

Time Comparison

Step	Traditional WB	2-Hour Rapid WB Kit	Time Saved
Total Duration	1-2 days	~2 hours	>90%
Electrophoresis	90min	45min	50%
Transfer	90min	25min	72%
Blocking	60min/Overnight	10min	83%
Primary Antibody Incubation	120min/Overnight	15min	88%
Secondary Antibody Incubation	60min	5min	92%
Washing & Detection	60min	10min	83%