EnkiLife 2-Hour Rapid Western Blot Ready-to-Use Complete Workflow Kit
A revolutionary Western Blot solution reducing experimental time to 2 hours with excellent reproducibility and reliable results
Product Overview
Western Blot (WB), as a core immunological experiment, often presents challenges due to complex workflows and inconsistent results. EnkiLife's new 2-Hour Rapid WB Ready-to-Use Complete Workflow Kit not only reduces experimental time to just 2 hours but also ensures highly reproducible results through innovative design.
This kit features standardized pre-cast gels and optimized formulations to eliminate batch variations and ensure consistency between experiments. It includes most reagents and consumables required for the complete workflow—users only need to supply water, ethanol/methanol, common antibodies, and related equipment.
Product Features
Ultra-Fast
Complete Western Blot workflow in approximately 2 hours, 5-10x faster than traditional methods
Optimized Workflow
Integrated solution from sample processing to detection—no additional reagents needed
Reliable Results
Excellent reproducibility with batch-to-batch consistency (CV <5%), minimal inter-experiment variation
Rapid Incubation
Proprietary blocking buffer and antibody diluent: 10-min blocking, 15-min primary antibody, 5-min secondary antibody
Ready-to-Use
Pre-cast gels, ready-to-use markers, pre-formulated buffers—use directly out of the box
Lab Safety
Pre-cast gels and odorless buffers prevent exposure to toxic reagents like acrylamide
Exceptional Reproducibility & Consistency
High Batch Consistency
Strict QC ensures consistent performance across batches (CV <5%)
Uniform Band Quality
Optimized pre-cast gel technology minimizes edge effects for sharper bands
Standardized Protocol
Simplified procedures reduce human error and improve reliability
Rigorous QC Standards
Every batch validated through Western Blot performance testing
Reproducibility Comparison: Band Intensity CV
Safety Features
Pre-Cast Gels
Safe polymerization technology eliminates exposure to neurotoxic reagents like acrylamide/TEMED
Odorless Buffers
Sample loading buffer excludes irritants like β-mercaptoethanol for improved lab air quality
Toxic Reagent Avoidance
Full workflow design prevents contact with hazardous chemicals common in traditional WB
Health Protection
Reduces researcher exposure risks and creates a safer lab environment
Kit Components
| Component | Size 2T(For 30 Tests) |
|---|---|
| Protein Loading Buffer (5X) | 0.2ml |
| Pre-stained Marker (10-180kDa) | 10μl |
| Pre-Cast Gel (Bis-Tris, 4-20%, 15 wells) | 2 units |
| Electrophoresis Buffer Granules | 2 packs (500ml/pack) |
| PVDF Membrane (0.45μm) | 2 sheets |
| Transfer Sponges (Filter-Free) | 4 pieces |
| Rapid Transfer Buffer Granules | 1 pack (1L/pack) |
| Protein-Free Rapid Blocking Buffer | 20ml |
| Rapid Antibody Diluent | 16ml |
| Rapid Wash Buffer (10X) | 14ml |
| ECL Substrate Solution A | 1.2ml |
| ECL Substrate Solution B | 1.2ml |
Storage Instructions:
Protein loading buffer and pre-stained marker: -20°C; other reagents: 4°C. Shelf life: 1 year. Full kit at 4°C: minimum 3 months stability.
Consistency Guarantee: All components pass strict QC with batch-to-batch CV <5%
Time Comparison
| Step | Traditional WB | 2-Hour Rapid WB Kit | Time Saved |
|---|---|---|---|
| Total Duration | 1-2 days | ~2 hours | >90% |
| Electrophoresis | 90min | 45min | 50% |
| Transfer | 90min | 25min | 72% |
| Blocking | 60min/Overnight | 10min | 83% |
| Primary Antibody Incubation | 120min/Overnight | 15min | 88% |
| Secondary Antibody Incubation | 60min | 5min | 92% |
| Washing & Detection | 60min | 10min | 83% |
Validation Data
Sample: Recombinant His-METRNL Protein (30.7kDa) | Loading: 80ng, 20ng, 10ng, 5ng, 1ng
Experimental Notes:
- Group A: Our kit results (triplicate experiments)
- Group B: Competitor kit results (triplicate experiments)
- Third-party validation for impartiality
- Protocols followed respective kit instructions
Conclusions:
- Cleaner background in Group A vs. Group B
- Sharper marker bands in Group A
- Higher sensitivity in Group A for low-abundance targets
- Band intensity CV of only 3.8% in Group A triplicates
Core Advantages
Enhanced Efficiency
Reduces 1-2 day protocols to 2 hours, accelerating research and lab output
Result Reproducibility
CV <5% ensures reliable data for publications and critical experiments
Improved Safety
Eliminates exposure to acrylamide and other hazardous chemicals
Cost Efficiency
Reduces reagent consumption and instrument time, lowering operational costs
Expert Support | Fast Shipping | Technical Guidance
