EnkiLife TSA Multiple Fluorescent Staining Kit
EnkiLife TSA Multiple Fluorescent Staining Kit is optimized for multiplex immunofluorescence (IF) on formalin-fixed paraffin-embedded (FFPE) sections, frozen sections, cultured cells (adherent or smear preparations), and organoid models.
The workflow is powered by tyramide signal amplification (TSA). Horseradish peroxidase (HRP) catalyzes the conversion of H₂O₂ into highly reactive oxygen radicals that covalently couple fluorophore-conjugated tyramides to electron-rich amino-acid residues (Trp, His, Tyr) in the immediate vicinity of the antigen-antibody complex.
Each tyramide is labeled with a spectrally distinct, photostable fluorophore, enabling iterative cycles of antibody binding, HRP-mediated amplification, and fluorophore deposition.
Product Advantages
7-Plex Signals from a Single Slide
7 spectrally resolved TSA fluorophores (480-750 nm) for any experimental panel
Flexible Antibody Selection
Compatible with primary antibodies from rabbit and mouse
Broad Applications
Works with FFPE sections, frozen sections, cells, and organoids
All-in-One Components
Complete kit with tyramides, buffers, reagents, and mounting medium
Gentle Antibody Elution
Special buffer removes antibodies while preserving antigens and tissue integrity
TSA Multiple Fluorescent Staining Kits
| Cat.# | Size | Product Name |
|---|---|---|
| RA10008 | 20T, 50T, 100T | 3-Color Multiple Fluorescent Staining Kit (mIHC) |
| RA10009 | 20T, 50T, 100T | 4-Color Multiple Fluorescent Staining Kit (mIHC) |
| RA10010 | 20T, 50T, 100T | 5-Color Multiple Fluorescent Staining Kit (mIHC) |
| RA10011 | 20T, 50T, 100T | 6-Color Multiple Fluorescent Staining Kit (mIHC) |
| RA10012 | 20T, 50T, 100T | 7-Color Multiple Fluorescent Staining Kit (mIHC) |
7-Color Multiple Fluorescent Staining Kit (mIHC)
Components and Dilutions
| Components | TSA-CY3 | TSA-FITC | TSA-680 | TSA-430 | TSA-594 | TSA-CY7 | TSA diluent | 3% H₂O₂ |
|---|---|---|---|---|---|---|---|---|
| Dilutions | 1:200 | 1:200 | 1:200 | 1:200 | 1:200 | 1:200 | Ready-to-use | Ready-to-use |
| Components | Blocking solution | Primary antibody diluent | Antibody elution reagent | HRP-goat anti-rabbit/mouse IgG | DAPI staining | Anti-fluorescence fading mounting medium |
|---|---|---|---|---|---|---|
| Dilutions | Ready-to-use | Ready-to-use | Ready-to-use | Ready-to-use | Ready-to-use | Ready-to-use |
Illustrative Images
Test Sample: Rat kidney
AQP4: Positive staining is evident in principal cells of the inner-medullary collecting ducts as well as in cortical and outer-medullary collecting ducts.
CD31: Widely used for labeling vascular endothelium; expressed in glomerular capillary endothelium and vessels at all hierarchical levels.
TGFβ1: Expression is markedly up-regulated in hypertension and fibrosis models; detected in glomerular epithelial cells (primarily podocytes) and interstitial fibroblasts.
Zo-1: As a scaffolding protein of tight junctions, it preserves the glomerular filtration barrier and tubular epithelial polarity.
Collagen I: In fibrotic or sclerotic models, it extensively accumulates in the interstitium and glomeruli.
| Target | Fluorescein | Ex (nm) | Em (nm) | Filters | Image Color |
|---|---|---|---|---|---|
| AQP4 | TSA-430 | 430nm | 480nm | Aqua channel | Yellow |
| CD31 | TSA-FITC | 490nm | 520nm | FITC channel | Green |
| TGFβ1 | TSA-CY3 | 550nm | 570nm | CY3 channel | Red |
| Zo-1 | TSA-594 | 590nm | 620nm | Red channel | Cyan |
| Collagen I | TSA-680 | 680nm | 700nm | CY5/CY5.5 channel | Purple |