PMS2 Rabbit Monoclonal Antibody
PMS2 Rabbit Monoclonal Antibody
Size:50μL Price:168 $99
Size:100μL Price:300 $180
Application:WB,IP
Reactivity:Human
Conjugate:Unconjugated
Optional conjugates: Biotin, FITC (free of charge). See other 26 conjugates.
Gene Name:PMS2
SKU: AMRe01493
Detail

Summary

Production Name

PMS2 Rabbit Monoclonal Antibody

Description

Recombinant rabbit monoclonal antibody

Host

Rabbit

Application

WB,IP

Reactivity

Human

 

Performance

Conjugation

Unconjugated

Modification

Unmodified

Isotype

IgG

Clonality

Monoclonal

Form

Liquid

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

Purification

Affinity Purification

 

Immunogen

Gene Name

PMS2

Alternative Names

DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2

Gene ID

5395

SwissProt ID

P54278

 

Application

Dilution Ratio

WB 1:500-1:1000,IP 1:20-1:50

Molecular Weight

Calculated MW: 96 kDa; Observed MW: 96 kDa

 

Background

Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.

 

Research Area

Epigenetics and Nuclear Signaling

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