Summary
| Production Name | Cleaved-Caspase 3 p12 Rabbit Monoclonal Antibody |
| Description | Recombinant rabbit monoclonal antibody |
| Host | Rabbit |
| Application | WB,ICC/IF |
| Reactivity | Human,Mouse,Rat |
Performance
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | IgG |
| Clonality | Monoclonal |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein |
| Purification | Affinity Purification |
Immunogen
| Gene Name | CASP3 |
| Alternative Names | CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1 |
| Gene ID | 836 |
| SwissProt ID | P42574 |
Application
| Dilution Ratio | WB 1:500-1:1000,ICC/IF 1:50-1:200 |
| Molecular Weight | Calculated MW: 32 kDa; Observed MW: 32,12 kDa |
Background
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
Research Area
Cell Biology