Basic Information

dsDNA Quantification Reagent (Green) excitation/emission wavelength: 480/520 nm

Product Introduction

dsDNA Quantification Reagent (Green) is a product for fluorescent detection and quantification of dsDNA, and this method is highly sensitive. It is commonly used in molecular biology techniques such as the construction of cDNA libraries, purification of subcloned DNA fragments, and applications like DNA quantification, product amplification, and further detection of primers. The conventional method for detecting DNA content is to measure its absorbance at 260 nm. The main drawbacks of this method are that nucleotides, single-stranded nucleic acids, and proteins can significantly affect the signal, and it is also susceptible to interference from contaminants in the nucleic acid preparation process. It cannot distinguish between DNA and RNA, and it is not sensitive (A260 = 0.1 for a 5 μg/mL dsDNA solution). The dsDNA Quantification Reagent (Green) method is simple and convenient, and has become the standard for residual DNA detection in biological products.

dsDNA Quantification Reagent (Green) only emits fluorescence after binding to dsDNA, and the intensity of the fluorescence is proportional to the DNA concentration. dsDNA Quantification Reagent (Green) can detect dsDNA in the range of 25 pg/mL to 1000 ng/mL with a good linear relationship (R2>0.99).

Reagent preparation

dsDNA Quantification Reagent (Green) is stored in anhydrous DMSO (dimethyl sulfoxide) as a 1 mL concentrate. During the experiment, prepare 2×dsDNA Quantification Reagent (Green) working solution: dilute the concentrate with 1×TE (10 mM Tris-HCl, 1 mM EDTA, pH 7.5) at a ratio of 1:200. For a final volume of 200 μL, if you need to prepare enough working solution for 20 samples, add 10 μL dsDNA Quantification Reagent (Green) to 1.99 mL 1×TE; for a final volume of 2 mL, if you need to prepare enough working solution for 20 samples, add 100 μL dsDNA Quantification Reagent (Green) concentrate to 19.9 mL 1×TE. Because the reagent is easily adsorbed to glass surfaces, it should be prepared in a plastic container. dsDNA Quantification Reagent (Green) is easily degraded by light, so it should be stored away from light.

Solutions are best used within a few hours of preparation to ensure best results.

Note: This reagent is for scientific research use only!