Summary
| Production Name | TriMethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody |
| Description | Recombinant rabbit monoclonal antibody |
| Host | Rabbit |
| Application | WB,IHC,ICC/IF,IP,ChIP |
| Reactivity | Human,Rat |
Performance
| Conjugation | Unconjugated |
| Modification | Methylated |
| Isotype | IgG |
| Clonality | Monoclonal |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein |
| Purification | Affinity Purification |
Immunogen
| Gene Name | H3C1 |
| Alternative Names | H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a |
| Gene ID | 8350 |
| SwissProt ID | P68431 |
Application
| Dilution Ratio | WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,IP 1:20-1:50,ChIP 1:20 |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
Background
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Research Area
Epigenetics and Nuclear Signaling