Product Introduction
The main sources of blood ammonia are endogenous ammonia and exogenous ammonia. Ammonia maintains a constant state in the blood, that is, the source and destination of blood ammonia maintain a dynamic balance. Ammonia is a toxic substance and is mainly metabolized and detoxified in the liver. When liver function is severely damaged, ammonia cannot be detoxified. Ammonia accumulates in the central nervous system, leading to hepatic encephalopathy.
Principle
This method is based on the principle of ammonia indophenol blue reaction. After precipitating the protein in serum (plasma) with a protein precipitant, the blood ammonia is determined using the phenol-hypochlorite direct colorimetric method. The generated blue indophenol is proportional to the concentration of ammonia and has a special absorption peak at 635nm. Based on this, the blood ammonia content in the sample can be calculated from the absorbance value.
Use protein precipitant to precipitate blood protein and destroy enzyme activity to prevent free ammonia from being produced after being separated from the body. At the same time, remove most of the interfering color substances. Use Berthelot reaction to form indigo in the protein-free filtrate. The color depth is proportional to the blood ammonia content. Compare with the standard solution to determine the blood ammonia content. The detection principle is as follows:
Components
No. | Components | Size (100T) | Storage |
Reagent 1 | Protein Precipitant I | 20 mL | -20℃, store at 2-8°C after opening. |
Reagent 2 | Protein Precipitant Ⅱ | 20 mL | -20℃, store at 2-8°C after opening. |
Reagent 3 | Color Developer I | 15 mL | -20℃, protect from light, store at 2-8°C after opening. |
Reagent 4 | Color Developer II | 15 mL | -20℃, protect from light, store at 2-8°C after opening. |
Reagent 5 | 7mmol/L Standard | 1.5 mL | -20℃, store at 2-8°C after opening. |
Consumable 1 | Microplate | 1 plate | RT |
Consumable 2 | Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20℃ for 12 months, and after opening, it can be stored at 2-8℃ for 3 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
