Product Introduction
Lactate Dehydrogenase (LDH) Activity Assay Kit, also known as LDH Cytotoxicity Assay Kit or LDH Release Assay Kit. The destruction of cell membrane structure caused by cell apoptosis or necrosis will lead to the release of enzymes in the cytoplasm into the culture medium, including LDH with relatively stable enzyme activity. By detecting the activity of LDH released into the culture medium from cells with ruptured plasma membranes, quantitative analysis of cytotoxicity can be achieved. LDH release is regarded as an important indicator of cell membrane integrity and is widely used in cytotoxicity detection.
Product Features
A wide range of detectable sample type: This assay kit is capable of detecting the activity of lactate dehydrogenase (LDH) in various samples such as cell culture supernatants and cell lysates. It is frequently used for cytotoxicity assays that take LDH release as an indicator. Moreover, based on the detection of total cellular LDH activity, this kit can also be used for the detection of cell proliferation and cytotoxicity.
Principle
Under the action of lactate dehydrogenase (LDH), NAD+ is reduced to generate NADH. NADH and INT (2-p-iodophenyl-3-nitrophenyl tetrazolium chloride) are then catalyzed by diaphorase to produce NAD+ and a strong-colored formazan. An absorption peak is generated at a wavelength of 490 nm, which allows for the quantification of LDH activity through colorimetry. The absorbance is linearly and directly proportional to the activity of lactate dehydrogenase. The schematic diagram of this enzyme-linked reaction principle is as follows:
Components
Components | Size (100T) | Size (500T) | Storage |
LDH Release Reagent | 1.5 ml | 7.5 ml | -20℃ |
Lactic Acid Solution | 1 ml/vial, 2 vials | 10 ml | -20℃ |
Enzyme Solution | 1 ml/vial, 2 vials | 10 ml | -20°C, avoid repeated freeze-thaw cycles. |
INT Solution (10×) | 0.2 ml | 0.2 ml | -20°C, protected from light. |
INT Dilution Buffer | 1 ml/vial, 2 vials | 1 ml/ vial, 2 vials | -20℃ |
Microplate | 1 plate | 5 plates | RT |
Plate Sealer | 2 pieces | 10 pieces | RT |
Storage
The unopened kit can be stored at -20°C for 12 months. After thawing, the kit can be stored at 2-8°C for a short period of time and is valid within 2-3 days.
Note
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
