Product Introduction
Acid phosphatase, also known as acid phosphatase, is an acid hydrolase with a high content in lysosomes and is considered to be a marker for identifying lysosomal subcellular components. Acid phosphatase is a family of proteins, and its molecular weight in mammals ranges from 18kD to 100kD. Acid phosphatases are divided into two categories, one is tartrate-sensitive and the other is fluoride-sensitive. Acid phosphatases in lysosomes are tartrate-sensitive, while acid phosphatases in red blood cells and macrophages are fluoride-sensitive. The activity of acid phosphatase in plasma ranges from 2-7.9U/L, and the activity of acid phosphatase in serum ranges from 2.5-11.7U/L. Semen contains high concentrations of acid phosphatase, and the activity can reach 87-436KU/L.
Detection principle
Para-nitrophenyl phosphate (pNPP) is a commonly used chromogenic substrate for phosphatase. Under acidic conditions, para-nitrophenol ( p -nitrophenol) can be generated by acid phosphatase. Under alkaline conditions, p -nitrophenol is a yellow product, and the absorbance can be detected at 400-415nm. The darker the yellow color of the product, the higher the acid phosphatase activity, and vice versa. Based on this, the acid phosphatase activity level can be calculated through colorimetric analysis.
Product composition
serial number | Product Name | Packing Specifications ( 120T ) | How to save |
Reagent 1 | Assay Buffer | 15ml | -20℃ |
Reagent 2 | Chromogenic substrate | 2 tubes | Store at -20℃, away from light . |
Reagent 3 | p -nitrophenol solution (10mM) | 0.1ml | Store at -20℃, away from light . |
Reagent 4 | Reaction stop solution | 20ml | -20℃ |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage conditions
The unopened kit can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
