Product Introduction
Sucrose is almost universally found in leaves, flowers, stems, seeds and fruits of the plant kingdom. It is particularly abundant in sugar cane, beets and maple sap.
Principle
Sucrose in plant tissues is hydrolyzed into glucose and fructose by heating in a boiling water bath under acidic conditions. Fructose loses water under acidic conditions to obtain 5-hydroxymethylfurfural. Glucose must first be isomerized into a ketose structure, and then loses water to obtain 5-hydroxymethylfurfural, but the rate of glucose isomerization to ketose is very slow. 5-Hydroxymethylfurfural has a maximum absorption peak at 290 nm. By measuring its OD value, the sucrose content is calculated. The figure below shows the standard curve for the determination of sucrose by this kit.
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | Hydrolyzate | 60 mL × 2 bottles | -20℃, avoid light, store at 2-8℃ after opening |
Reagent 2 | 100 μmol/mL Sucrose Standard | 1 mL × 1 tube | -20℃, avoid light, store at 2-8℃ after opening |
Storage
The unopened test kit can be stored at -20℃ for 12 months. After opening, it can be stored at 2-8℃ away from light for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
