Product Introduction
Nitrite is widely present in the human environment and is the most common nitrogen-containing compound in nature.
Detection principle
NO 2- reacts with the color developer to generate a light red azo compound. The concentration of the generated azo compound has a linear relationship with the concentration of NO 2- . The concentration of NO 2- can be determined by colorimetry . The role of reagents 1 and 2 is to remove the interference of colored substances in the sample. There is a maximum absorption peak at 550nm, and the absorbance value is proportional to the concentration within a certain range.
When this kit is used to test tissue samples, the total protein concentration needs to be measured. We recommend the protein concentration determination kit (BCA method) (BC00006) produced by EnkiLife.
The figure below shows the standard curve for the determination of nitrite by this kit. The following standard curve is for reference only:
Product composition
Serial Number | Product Name | Packing Specifications (100T) | Storage |
Reagent 1 | Saline solution | 50 mL×2 bottles | store at 2-8℃ after opening |
Reagent 2 | Alkaline solution | 50 mL×1 bottle | store at 2-8℃ after opening |
Reagent 3 | Chromogen A | Powder × 1 | store at 2-8℃ after opening |
Reagent 4 | Chromogen B | Powder × 1 | store at 2-8℃ after opening |
Reagent 5 | Acid solution | 12 mL×1 bottle | store at 2-8℃ after opening |
Reagent VI | Sodium Nitrite Standard | Powder × 1 | -20℃ |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Note: The reagents must be stored strictly according to the storage conditions in the table above. Reagents from different test kits cannot be mixed. For reagents with a small volume, please centrifuge before use to avoid insufficient amount of reagent.
Storage conditions
The unopened kit can be stored at -20℃ for 12 months. After opening, it can be stored at 4℃ for 6 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
