Product Introduction
Vitamin C, as a key antioxidant, plays multiple physiological roles in the body, including promoting collagen synthesis, enhancing iron absorption, and neutralizing free radicals. Accurate determination of vitamin C levels is essential for assessing an individual's nutritional status and overall antioxidant capacity.
Detection Principle
In the presence of phenanthroline, VC can reduce trivalent iron ions to divalent iron ions, which can form a pink complex with phenanthroline under certain conditions, with a maximum absorption wavelength at 536 nm. The content of VC can be calculated by colorimetry.
When this kit is used to test tissue samples, the total protein concentration needs to be measured. We recommend the protein concentration determination kit (BCA method) (BC00006) produced by EnkiLife.
Product composition
Serial Number | Product Name | Packing specification ( 50T ) | Storage |
Reagent 1 | Extract | 20 mL | -20℃, avoid light, store at 2-8℃ after opening |
Reagent 2 | Buffer | 8 mL | -20℃, store at 2-8℃ after opening |
Reagent 3 | Color developer | 15 mL | -20℃, avoid light, store at 2-8℃ after opening |
Reagent 4 | Iron reagent | 0.3 mL | -20℃, avoid light, store at 2-8℃ after opening |
Reagent 5 | VC Standard | 6 mg | -20℃, avoid light, store at 2-8℃ after opening |
Consumables 1 | 96-well ELISA plate | 1 plate | RT |
Consumables 2 | 96-well membrane | 2 pieces | RT |
Storage conditions
The unopened kit can be stored at -20℃ for 12 months. After opening, it can be stored at 4℃ for 6 months.
Notes
This product is limited to scientific research by professionals and must not be used for clinical diagnosis or treatment, used as food or medicine, or stored in ordinary residences.